CBP3 / YPL215W Overview
- Standard Name
- CBP3 1
- Systematic Name
- YPL215W
- SGD ID
- SGD:S000006136
- Feature Type
- ORF , Verified
- Description
- Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation 2 3 4 5
- Name Description
- Cytochrome B mRNA Processing 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 335
- Mol. Weight (Da)
- 39096.2
- Isoelectric Point
- 9.78
- Median Abundance (molecules/cell)
- 3987 +/- 1030
- Half-life (hr)
- 6.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CBP3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein that binds to the mitochondrial ribosome and to COB mRNA; component of the Cbp3p-Cbp6p complex involved in mitochondrial cytochrome bc(1) complex assembly and mitochondrial translation
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (IDA)
- contributes to ribosome binding (IPI, IDA)
Biological Process
- Manually Curated
- involved in mitochondrial respiratory chain complex III assembly (IMP)
- involved in positive regulation of mitochondrial translation (IMP)
Cellular Component
- Manually Curated
- part of Cbp3p-Cbp6 complex (IPI)
- located in mitochondrial membrane (IDA)
- located in mitochondrial ribosome (IDA)
- located in mitochondrion (HDA, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; in systematic studies, null mutants are incapable of respiratory growth on non-fermentable carbon sources, grow slowly on fermentable carbon sources and show reduced competitive fitness
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- heat sensitivity: decreased
- innate thermotolerance: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- sporulation: abnormal
- starvation resistance: decreased
- stress resistance: increased
- utilization of carbon source: decreased
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
107 total interactions for 81 unique genes
Physical Interactions
- Affinity Capture-MS: 28
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 14
- Co-crystal Structure: 2
- Co-fractionation: 6
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 2
- Proximity Label-MS: 36
- Two-hybrid: 3
Genetic Interactions
- Negative Genetic: 1
- Phenotypic Suppression: 1
- Synthetic Growth Defect: 3
- Synthetic Lethality: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.