FOB1 / YDR110W Overview
- Standard Name
- FOB1 1
- Systematic Name
- YDR110W
- SGD ID
- SGD:S000002517
- Aliases
- HRM1 2
- Feature Type
- ORF , Verified
- Description
- Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking and recombinational hotspot activity that results in concerted evolution of the rDNA, termed homogenization; role in recruitment of condensin to the replication fork barrier (RFB), contributing to the faithful segregation of rDNA repeats; proposed role in recovery from low rDNA copy number; related to retroviral integrases 1 2 3 4 5 6 7
- Name Description
- FOrk Blocking less 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 566
- Mol. Weight (Da)
- 65309.2
- Isoelectric Point
- 6.1
- Median Abundance (molecules/cell)
- 1687 +/- 729
- Half-life (hr)
- 7.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FOB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- rDNA spacer replication fork barrier-binding protein; involved in replication fork arrest at rDNA repeats, chromatin silencing at rDNA, rDNA condensation, and maintenance of rDNA; also involved in replicative cell aging, extrachromosomal circular DNA accumulation during aging, dsDNA loop formation, and regulation of DNA replication and recombination; localizes to rDNA heterochromatin and the nucleolus
View computational annotations
Molecular Function
- Manually Curated
- enables rDNA spacer replication fork barrier binding (IDA, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in chromatin organization (IGI)
- involved in chromosome segregation (IGI)
- involved in DNA recombination (IMP)
- involved in maintenance of DNA repeat elements (IMP)
- involved in maintenance of rDNA (IGI)
- involved in negative regulation of DNA replication (IDA)
- involved in positive regulation of DNA recombination (IMP)
- involved in protein localization to nucleolar rDNA repeats (IMP)
- involved in rDNA chromatin condensation (IMP)
- involved in rDNA heterochromatin formation (IDA, IMP)
- involved in replication fork arrest at rDNA repeats (IMP)
Cellular Component
- Manually Curated
- located in nucleolus (IDA)
- located in rDNA heterochromatin (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits lengthened replicative lifespan, increased resistance to MMS, and reduced Hot1p-mediated mitotic recombination; in large-scale studies, null mutant displays increased rDNA recombination during meiosis but decreased rDNA recombination during mitosis; null mutant shows reduced starvation resistance and competitive fitness
Classical Genetics
- null
- chromosome/plasmid maintenance: decreased
- meiotic recombination: increased
- mitotic recombination: decreased
- mitotic recombination: decreased rate
- protein/peptide distribution: abnormal
- replicative lifespan: increased
- resistance to chemicals: increased
- sporulation efficiency: increased
- viability: increased
- overexpression
- unspecified
- reduction of function
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
218 total interactions for 130 unique genes
Physical Interactions
- Affinity Capture-MS: 27
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 9
- Co-localization: 5
- Co-purification: 1
- Reconstituted Complex: 4
- Two-hybrid: 23
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 1
- Negative Genetic: 40
- Phenotypic Enhancement: 14
- Phenotypic Suppression: 54
- Positive Genetic: 2
- Synthetic Growth Defect: 7
- Synthetic Lethality: 5
- Synthetic Rescue: 20
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 1
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.