FUS3 / YBL016W Overview
- Standard Name
- FUS3 1
- Systematic Name
- YBL016W
- SGD ID
- SGD:S000000112
- Aliases
- DAC2 28
- Feature Type
- ORF , Verified
- Description
- Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis 2 3 4 5 6 7 8
- Name Description
- cell FUSion 9
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Fus3p is 353 amino acids long, short-lived, low in abundance; phosphorylated on 8 residues
- Length (a.a.)
- 353
- Mol. Weight (Da)
- 40770.5
- Isoelectric Point
- 7.13
- Median Abundance (molecules/cell)
- 4800 +/- 1651
- Half-life (hr)
- 4.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FUS3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- MAP kinase involved in pheromone-dependent signal transduction during mating; has a role in invasive growth in response to glucose limitation and negative regulation of MAPK cascade; localized to both the nucleus and mating projection tip
View computational annotations
Molecular Function
- Manually Curated
- enables MAP kinase activity (IDA)
- enables protein kinase activity (HDA)
Biological Process
- Manually Curated
- involved in invasive growth in response to glucose limitation (IMP)
- involved in negative regulation of MAPK cascade (IPI)
- involved in pheromone response MAPK cascade (IDA)
- involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion (IDA)
- involved in positive regulation of protein export from nucleus (IMP)
- involved in response to pheromone triggering conjugation with cellular fusion (IDA)
- involved in transposable element silencing (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytoplasmic stress granule (HDA)
- located in mating projection tip (IDA)
- located in mitochondrion (HDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; isolated based on a bilateral cell fusion defect; null mutant has reduced mating efficiency and is unable to undergo pheromone induced G1 arrest; overexpression increases pheromone sensitivity; null mutant displays decreased autophagy and endocytosis; null mutant is hyperfilamentous and disrupts the nuclear localization of Sks1p and Ksp1p after induction of filamentous growth
Classical Genetics
- null
- autophagy: decreased
- cell death: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- endocytosis: decreased
- filamentous growth: increased
- mating efficiency: decreased
- necrotic cell death: decreased
- oxidative stress resistance: decreased
- pheromone-induced cell cycle arrest: absent
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- sterile
- survival rate in stationary phase: decreased
- transposable element transposition: increased
- overexpression
- reduction of function
- unspecified
- gain of function
- conditional
- overexpression
- null
- bud morphology: abnormal
- chemical compound accumulation: abnormal
- chronological lifespan: decreased
- endocytosis: decreased
- heat sensitivity: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- metal resistance: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vacuolar morphology: abnormal
- viable
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fus3p interacts physically with proteins involved in chromatin organization; FUS3 interacts genetically with genes involved in transcription
646 total interactions for 439 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 26
- Biochemical Activity: 108
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 2
- Co-purification: 1
- FRET: 7
- PCA: 2
- Reconstituted Complex: 31
- Two-hybrid: 23
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Rescue: 4
- Negative Genetic: 146
- Phenotypic Enhancement: 25
- Phenotypic Suppression: 29
- Positive Genetic: 172
- Synthetic Growth Defect: 10
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 2
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-07-04
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.