MRE11 / YMR224C Overview
- Standard Name
- MRE11 1
- Systematic Name
- YMR224C
- SGD ID
- SGD:S000004837
- Aliases
- RAD58 , XRS4 , NGS1 28
- Feature Type
- ORF , Verified
- Description
- Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress 2 3 4 6 7
- Name Description
- Meiotic REcombination 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 692
- Mol. Weight (Da)
- 77633.3
- Isoelectric Point
- 5.66
- Median Abundance (molecules/cell)
- 888 +/- 400
- Half-life (hr)
- 20.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MRE11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Nuclease subunit of the Mre11 complex; has endonuclease and 3-5 exonuclease activity; binds single and double-stranded telomeric DNA and G-quadruplex DNA; involved in multiple processes during meiosis including reciprocal recombination, DNA double-strand break formation and processing, and regulation of transcription; involved in various DNA repair processes such as base-excision repair and double-strand break repair via nonhomologous end joining or break-induced replication; also involved in maintenance of DNA trinucleotide repeats and telomeres
View computational annotations
Molecular Function
- Manually Curated
- enables 3'-5' exonuclease activity (IDA)
- contributes to adenylate kinase activity (IDA)
- enables DNA endonuclease activity (IDA)
- enables double-stranded telomeric DNA binding (IDA)
- enables endonuclease activity (IDA)
- enables G-quadruplex DNA binding (IDA)
- enables molecular adaptor activity (IMP, IGI)
- enables single-stranded telomeric DNA binding (IDA)
- enables telomeric DNA binding (IDA)
Biological Process
- Manually Curated
- involved in ascospore formation (IMP)
- involved in base-excision repair (IMP)
- involved in DNA double-strand break processing involved in repair via synthesis-dependent strand annealing (IMP)
- involved in DNA repair (IMP)
- involved in double-strand break repair via break-induced replication (IGI, IMP)
- involved in double-strand break repair via nonhomologous end joining (IMP)
- involved in maintenance of DNA trinucleotide repeats (IMP)
- involved in meiotic cell cycle (IMP)
- involved in mitochondrial double-strand break repair via homologous recombination (IMP)
- involved in reciprocal meiotic recombination (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in telomere maintenance (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
- part of Mre11 complex (IPI)
- located in nucleus (IDA, HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows defects in chromosome maintenance, elevated mutation frequency and sensitivity to gamma radiation; null and reduction-of-function mutations increase sensitivity to DNA-affecting chemicals, such as MMS, cisplatin, daunorubicin, hydroxyurea, camptothecin; null mutation leads to shortened telomeres and increased Ty element transposition
Classical Genetics
- null
- apoptosis: increased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- endoplasmic reticulum morphology: abnormal
- gamma ray resistance: decreased
- metal resistance: decreased
- mitochondrial genome maintenance: abnormal
- mutation frequency: increased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: normal
- RNA accumulation: increased
- spore wall formation: abnormal
- telomere length: decreased
- transposable element transposition: increased
- vegetative growth: decreased
- unspecified
- reduction of function
- gain of function
- null
- acid pH resistance: decreased
- chitin deposition: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- fermentative growth: decreased rate
- haploinsufficient
- haploproficient
- heat sensitivity: increased
- metal resistance: decreased
- mutation frequency: increased
- nutrient utilization: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- sporulation: decreased
- telomere length: decreased
- toxin resistance: decreased
- toxin resistance: increased
- transposable element transposition: increased
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- unspecified
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MRE11 is homologous to human MRE11, and is used for functional screening of human cancer mutations, including those implicated in breast, liver, skin, intestinal, and ovarian cancers
Manually Curated
- breast cancer (IGI, ISS, IMP)
- intestinal cancer (ISS, IMP)
- liver cancer (ISS, IMP)
- ovarian cancer (ISS, IMP)
- skin cancer (ISS, IMP)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1376 total interactions for 633 unique genes
Physical Interactions
- Affinity Capture-MS: 13
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 50
- Biochemical Activity: 1
- Co-fractionation: 1
- Co-localization: 4
- Co-purification: 5
- Far Western: 2
- Protein-peptide: 2
- Reconstituted Complex: 22
- Two-hybrid: 43
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Lethality: 3
- Dosage Rescue: 9
- Negative Genetic: 541
- Phenotypic Enhancement: 79
- Phenotypic Suppression: 65
- Positive Genetic: 49
- Synthetic Growth Defect: 215
- Synthetic Lethality: 222
- Synthetic Rescue: 39
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-02-28
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.