NGG1 / YDR176W Overview
- Standard Name
- NGG1 1
- Systematic Name
- YDR176W
- SGD ID
- SGD:S000002583
- Aliases
- ADA3 19 , SWI7 20
- Feature Type
- ORF , Verified
- Description
- Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes 1 2 3 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 702
- Mol. Weight (Da)
- 79257.9
- Isoelectric Point
- 4.75
- Median Abundance (molecules/cell)
- 2457 +/- 349
- Half-life (hr)
- 9.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NGG1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the SAGA, SLIK, and Ada2/Gcn5/Ada3 histone acetyltransferase complexes involved in chromatin modification and transcription activation
View computational annotations
Molecular Function
- Manually Curated
- contributes to histone acetyltransferase activity (IDA)
Biological Process
- Manually Curated
- involved in chromatin organization (IDA)
Cellular Component
- Manually Curated
- part of ADA complex (IDA)
- part of SAGA complex (IDA)
- part of SLIK (SAGA-like) complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, have shortened lifespan, decreased fitness and ethanol tolerance, abnormal morphology of vacuoles and buds, are sensitive to preservatives, antibiotics, heat, osmotic stress, dessication; homozygous diploid nulls cannot sporulate; overexpression affects chromatin silencing
Classical Genetics
- null
- heat sensitivity: increased
- metal resistance: decreased
- osmotic stress resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide modification: decreased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- vegetative growth: decreased rate
- null
- alkaline pH resistance: decreased
- auxotrophy
- bud morphology: abnormal
- cell cycle progression in G1 phase: delayed
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- endoplasmic reticulum morphology: abnormal
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- metal resistance: increased
- mitotic recombination: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- respiratory growth: increased
- sporulation: absent
- transposable element transposition: increased
- utilization of carbon source: decreased
- utilization of iron source: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
463 total interactions for 179 unique genes
Physical Interactions
- Affinity Capture-MS: 134
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 81
- Biochemical Activity: 1
- Co-fractionation: 6
- Co-purification: 45
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 11
- Two-hybrid: 14
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 68
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 6
- Synthetic Growth Defect: 66
- Synthetic Lethality: 19
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- NGG1 (also called ADA3) encodes a component of three chromatin modifying histone acetyltransferase (HAT) complexes: SAGA, SLIK, and ADA. These complexes function in positive and negative transcriptional regulation of numerous RNA polymerase II-transcribed genes; in addition, SLIK plays a role in the retrograde response. The three complexes each contain the histone acetyltransferase catalytic subunit Gcn5p, which interacts directly with Ada2p and preferentially modifies histones H3 and H2B. In vitro, Gcn5p acetylates N-terminal lysines on free histones, but acetylation of nucleosomal histone substrates also requires Ada2p and Ngg1p, which are found in a complex with Gcn5p. Ada2p has been shown to increase the HAT activity of Gcn5p, while Ngg1p plays a role in expanding the range of lysines that undergo acetylation.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-03-26
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.