NUT2 / YPR168W Overview
- Standard Name
- NUT2 1
- Systematic Name
- YPR168W
- SGD ID
- SGD:S000006372
- Aliases
- MED10
- Feature Type
- ORF , Verified
- Description
- Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress 2 3 4
- Name Description
- Negative regulation of URS Two 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 157
- Mol. Weight (Da)
- 17901.1
- Isoelectric Point
- 4.62
- Median Abundance (molecules/cell)
- 2953 +/- 1240
- Half-life (hr)
- 8.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NUT2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the core mediator complex, which is involved in assembly of RNA polymerase II preinitiation complex and regulation of transcription by RNA polymerase II; localized to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in negative regulation of transcription by RNA polymerase II (IGI, IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in RNA polymerase II preinitiation complex assembly (IMP)
Cellular Component
- Manually Curated
- part of core mediator complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; reduction of function decreases competitive fitness; conditional (ts) mutation results in reduced size of nucleolus
Classical Genetics
- null
- reduction of function
- reduction of function
- null
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
468 total interactions for 350 unique genes
Physical Interactions
- Affinity Capture-MS: 67
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 16
- Biochemical Activity: 1
- Co-purification: 1
- PCA: 4
- Reconstituted Complex: 11
- Two-hybrid: 19
Genetic Interactions
- Negative Genetic: 273
- Phenotypic Suppression: 2
- Positive Genetic: 61
- Synthetic Growth Defect: 1
- Synthetic Lethality: 3
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- NUT2 (also known as MED10) encodes a component of the middle module of the Mediator transcriptional regulation complex. The Mediator complex is highly conserved from yeast to human, and is a central player in the transcriptional regulation of protein-coding genes, being required for nearly all transcription by RNA polymerase II (RNAPII). Mediator bridges activators and the general transcription machinery. The core Mediator complex contains three distinct head, middle, and tail modules. Other subunits of the middle module are encoded by MED1, MED4, MED7, CSE2/MED9, RGR1/MED14, SRB7/MED21, and SOH1/MED31. The tail module interacts with gene expression regulators, while the head and middle modules interact with general transcription factors and RNAPII subunits to regulate transcription initiation. Nut2p bridges between the middle and tail modules, and within the middle module binds Med4p and Med7p.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.