PKH1 / YDR490C Overview
- Standard Name
- PKH1 1
- Systematic Name
- YDR490C
- SGD ID
- SGD:S000002898
- Feature Type
- ORF , Verified
- Description
- Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ypk2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication 1 2 3 4 5 6
- Name Description
- Pkb-activating Kinase Homolog 1
- Paralog
- PKH2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- PKH1 has a paralog, PKH2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Contains a PH-like domain
- Length (a.a.)
- 766
- Mol. Weight (Da)
- 86272.6
- Isoelectric Point
- 9.76
- Median Abundance (molecules/cell)
- 1767 +/- 422
- Half-life (hr)
- 10.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PKH1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Serine/threonine protein kinase; component of the MAPK cascade that regulates the cell wall integrity pathway by activating Ypk1p; regulates late endosome to vacuole transport via the MVB sorting pathway through Vps27p phosphorylation; regulates assembly of the cytoplasmic mRNA processing body (P-body) and mRNA decay via poly(A) tail shortening; involved in Rps6a/b phosphorylation; localizes to the cell cortex, the cytosol and colocalizes with eicosomes
View computational annotations
Molecular Function
- Manually Curated
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in cell integrity MAPK cascade (IGI, IMP)
- involved in endocytosis (IMP)
- involved in late endosome to vacuole transport via multivesicular body sorting pathway (IGI)
- involved in positive regulation of cytoplasmic mRNA processing body assembly (IGI)
- involved in regulation of nuclear-transcribed mRNA poly(A) tail shortening (IGI)
Cellular Component
- Manually Curated
- located in cell cortex (IDA)
- located in cytosol (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays sensitivity to multiple drug,including caffeine, fluconazole, tunicamycin, hydroxyurea, bleomycin, MMS, cicloheximide
Classical Genetics
- null
- overexpression
- null
- chemical compound accumulation: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- innate thermotolerance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The pkh1 null mutant is viable; the null mutant of paralog pkh2 is inviable in the CEN.PK background, but viable in the S288C background; the pkh1 pkh2 double mutant is inviable or displays a growth defect.
216 total interactions for 156 unique genes
Physical Interactions
- Affinity Capture-MS: 31
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 4
- Biochemical Activity: 12
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 1
- PCA: 2
- Reconstituted Complex: 11
- Two-hybrid: 5
Genetic Interactions
- Dosage Growth Defect: 7
- Dosage Rescue: 1
- Negative Genetic: 84
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 2
- Positive Genetic: 20
- Synthetic Growth Defect: 22
- Synthetic Lethality: 4
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 11
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.