POL2 / YNL262W Overview
- Standard Name
- POL2 1
- Systematic Name
- YNL262W
- SGD ID
- SGD:S000005206
- Aliases
- DUN2 8 , Pol ε
- Feature Type
- ORF , Verified
- Description
- Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p 2 3 4 5 6 7
- Name Description
- POLymerase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- POL2/YNL262W is located on the left arm of chromosome XIV between YIF1 integral membrane protein and ORC5 origin recognition complex subunit; coding sequence is 6669 nucleotides long with 68 SNPs, 13 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pol2p is 2222 amino acids long, low in abundance; acetylated on 3 residues, sumoylated on K571, ubiquitinylated on K1063, phosphorylated on 10 residues
- Length (a.a.)
- 2222
- Mol. Weight (Da)
- 255663.5
- Isoelectric Point
- 6.94
- Median Abundance (molecules/cell)
- 3153 +/- 1183
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all POL2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-directed DNA polymerase and single-stranded DNA 3'-5' exodeoxyribonuclease; subunit of the epsilon DNA polymerase complex; involved in DNA-dependent DNA replication, leading strand elongation, DNA replication proofreading, and DNA repair; also involved in mitotic sister chromatid cohesion, heterochromatin organization involved in chromatin silencing, the mitotic DNA replication checkpoint, and the intra-S DNA damage checkpoint
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-directed DNA polymerase activity (IMP, IDA)
- contributes to double-stranded DNA binding (IDA)
- enables mRNA binding (HDA)
- enables single-stranded DNA 3'-5' DNA exonuclease activity (IMP)
- enables single-stranded DNA binding (IDA)
- enables SUMO binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in base-excision repair (IMP)
- involved in DNA replication proofreading (IMP)
- involved in DNA-templated DNA replication (IDA)
- involved in double-strand break repair (IMP)
- involved in double-strand break repair via nonhomologous end joining (IMP, IGI)
- involved in error-prone translesion synthesis (IDA)
- involved in gene conversion (IMP)
- involved in leading strand elongation (IMP)
- involved in mitotic DNA replication checkpoint signaling (IMP)
- involved in mitotic intra-S DNA damage checkpoint signaling (IGI, IMP, IPI)
- involved in mitotic sister chromatid cohesion (IMP)
- involved in nucleotide-excision repair, DNA gap filling (IMP)
Cellular Component
- Manually Curated
- part of epsilon DNA polymerase complex (IDA)
- located in replication fork (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; heat-sensitive mutant arrests as large-budded cells with nuclei at the mother-bud neck; point mutations affect chromosome stability and mutation frequency, and confer sensitivity to HU, MMS, and camptothecin; in large-scale studies, mutants display reduced competitive fitness and an Opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline)
Classical Genetics
- repressible
- reduction of function
- null
- conditional
- unspecified
- null
- repressible
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast POL2 is homologous to human POLE, and has been used to study mutations found in patients with hypermutable colorectal and endometrial cancers, anaplastic astrocytoma, breast carcinoma, duodenal cancer, glioblastoma, high grade glioma, kidney cancer, liver cancer, lung cancer, ovarian cancer, prostate cancer, pleomorphic xanthoastrocytoma, stomach adenocarcinoma, and uterine serous carcinoma
Manually Curated
- anaplastic astrocytoma (ISS, IMP)
- breast carcinoma (ISS, IMP)
- cancer (ISS, IMP)
- colorectal cancer (ISS, IMP)
- duodenum cancer (ISS, IMP)
- endometrial cancer (ISS, IMP)
- endometrial serous adenocarcinoma (ISS, IMP)
- gastric adenocarcinoma (ISS, IMP)
- glioblastoma (ISS, IMP)
- high grade glioma (ISS, IMP)
- kidney cancer (ISS, IMP)
- liver cancer (ISS, IMP)
- lung cancer (ISS, IMP)
- ovarian cancer (ISS, IMP)
- pleomorphic xanthoastrocytoma (ISS, IMP)
- prostate cancer (ISS, IMP)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pol2p interacts physically with proteins involved in DNA repair; POL2 interacts genetically with genes involved in DNA repair
973 total interactions for 639 unique genes
Physical Interactions
- Affinity Capture-MS: 67
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 52
- Biochemical Activity: 13
- Co-crystal Structure: 2
- Co-fractionation: 3
- Co-localization: 1
- Co-purification: 16
- Proximity Label-MS: 1
- Reconstituted Complex: 26
- Two-hybrid: 25
Genetic Interactions
- Dosage Rescue: 13
- Negative Genetic: 243
- Phenotypic Enhancement: 55
- Phenotypic Suppression: 23
- Positive Genetic: 67
- Synthetic Growth Defect: 202
- Synthetic Lethality: 46
- Synthetic Rescue: 110
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- POL2 promoter is bound by Reb1p, Stp1p, Uga3p, and Yap5p in response to heat; POL2 transcription is regulated by Sfp1p in response to stress; POL2 transcription is upregulated by Znf1p during xylose fermentation
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.