RIM15 / YFL033C Overview
- Standard Name
- RIM15 1
- Systematic Name
- YFL033C
- SGD ID
- SGD:S000001861
- Aliases
- TAK1
- Feature Type
- ORF , Verified
- Description
- Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase 2 3 4 5 6 7
- Name Description
- Regulator of IME2 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1770
- Mol. Weight (Da)
- 196508.3
- Isoelectric Point
- 6.43
- Median Abundance (molecules/cell)
- 1302 +/- 613
- Half-life (hr)
- 5.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RIM15 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase that positively regulates mitotic cell cycle phase transition, meiotic transcription, and autophagy; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (IDA, HDA)
- enables protein serine/threonine kinase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in cellular response to heat (IMP)
- acts upstream of or within cellular response to hydrogen peroxide (IMP)
- involved in cellular response to nitrogen starvation (IMP, IGI)
- acts upstream of or within cellular response to oxidative stress (IMP)
- involved in meiotic cell cycle (IMP)
- involved in positive regulation of autophagy (IMP, IGI)
- involved in positive regulation of G1 to G0 transition (IGI, IMP)
- involved in positive regulation of mitotic cell cycle phase transition (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation shortens chronological lifespan, causes increased apoptosis and decreased autophagy in response to nitrogen starvation; null mutant shows increased rate of fermentative growth, lower resistance to hyperosmotic stress and decreased innate thermotolerance; homozygous null mutant diploid shows lower sporulation efficiency and decreased growth rate on non-fermentable carbon sources (glycerol or ethanol); in systematic studies null mutants are sensitive to rapamycin and flucytosine
Classical Genetics
- null
- apoptosis: increased
- autophagy: decreased
- budding index: increased
- cell cycle progression in G1 phase: increased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- desiccation resistance: absent
- desiccation resistance: decreased
- entry into G0 (stationary phase): decreased
- fermentative growth: increased rate
- fermentative metabolism: increased
- growth in exponential phase: increased rate
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- metal resistance: decreased
- metal resistance: increased
- nuclear import: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide modification: decreased
- RNA accumulation: decreased
- sporulation efficiency: decreased
- utilization of carbon source: decreased
- utilization of nitrogen source: decreased
- viability: decreased
- unspecified
- repressible
- overexpression
- reduction of function
- null
- chemical compound accumulation: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- endocytosis: decreased
- haploproficient
- innate thermotolerance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- reticulophagy: decreased
- sporulation efficiency: decreased
- sporulation: decreased
- vacuolar morphology: abnormal
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
619 total interactions for 394 unique genes
Physical Interactions
- Affinity Capture-MS: 17
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 7
- Biochemical Activity: 26
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 6
- Negative Genetic: 426
- Phenotypic Enhancement: 12
- Phenotypic Suppression: 25
- Positive Genetic: 62
- Synthetic Growth Defect: 5
- Synthetic Lethality: 2
- Synthetic Rescue: 17
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.