SWE1 / YJL187C Overview
- Standard Name
- SWE1 1
- Systematic Name
- YJL187C
- SGD ID
- SGD:S000003723
- Aliases
- WEE1 1
- Feature Type
- ORF , Verified
- Description
- Protein kinase that regulates the G2/M transition; negative regulator of the Cdc28p kinase; morphogenesis checkpoint kinase; positive regulator of sphingolipid biosynthesis via Orm2p; phosphorylates a tyrosine residue in the N-terminus of Hsp90 in a cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate 1 2 3 4 5
- Name Description
- Saccharomyces WEe1 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 819
- Mol. Weight (Da)
- 92452.9
- Isoelectric Point
- 6.2
- Median Abundance (molecules/cell)
- 1047 +/- 579
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SWE1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Tyrosine kinase; involved in G2/M transition of mitotic cell cycle, G2 cell size control checkpoint, re-entry into the mitotic cell cycle, negative regulation of spindle pole body separation and meiosis; positive regulator of sphingolipid biosynthesis; localizes to the bud neck and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (HDA)
- enables protein tyrosine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IMP, IDA)
- involved in mitotic morphogenesis checkpoint signaling (IMP, IDA)
- involved in negative regulation of mitotic spindle pole body separation (IMP, IGI)
- involved in positive regulation of sphingolipid biosynthetic process (IGI, IMP)
- involved in re-entry into mitotic cell cycle (IGI)
- involved in regulation of cell size (HMP)
- involved in regulation of meiotic nuclear division (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant udergoes premature G2/M phase transition in the cell cycle; null mutation causes reduction in sphingoid long-chain bases, such as phytosphingosine; overexpression leads to elongated cells and G2 phase cell cycle arrest
Classical Genetics
- null
- bud morphology: abnormal
- bud morphology: normal
- cell cycle progression through the G2/M phase transition: increased
- cell cycle progression through the G2/M phase transition: premature
- cell shape: abnormal
- cell size: decreased
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- chromosome/plasmid maintenance: decreased rate
- mitotic recombination: increased rate
- position of spindle pole body: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- overexpression
- null
- cell size: decreased
- colony sectoring: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploproficient
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- toxin resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
725 total interactions for 465 unique genes
Physical Interactions
- Affinity Capture-MS: 31
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 23
- Biochemical Activity: 131
- Co-localization: 1
- PCA: 1
- Protein-RNA: 1
- Reconstituted Complex: 11
- Two-hybrid: 21
Genetic Interactions
- Dosage Growth Defect: 28
- Dosage Lethality: 2
- Dosage Rescue: 8
- Negative Genetic: 230
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 62
- Positive Genetic: 47
- Synthetic Growth Defect: 16
- Synthetic Lethality: 18
- Synthetic Rescue: 59
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2002-03-20
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.