Methods are described for the partial purification of the Saccharomyces cerevisiae class III gene transcription factor TFIIIB from yeast whole cell extracts. A major component (30% of the total protein) of our most highly purified TFIIIB preparation was a polypeptide with an apparent Mr = 60,000 when analyzed by denaturing polyacrylamide gel electrophoresis. This protein was purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and polyclonal antibodies were raised against it. Using immunological methods it was shown that TFIIIB transcription factor activity was associated with this purified polypeptide. Furthermore, the polyclonal sera raised against the yeast TFIIIB polypeptide was also shown to specifically neutralize the activity of the human TFIIIB equivalent when it was added to a human KB cell S-100 in vitro transcription system.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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