The subunit dissociation of oligomeric enzymes is a major challenge that limits their practical applications. In this study, yeast-surface-displayed tetrameric β-glucuronidase with a C-terminal anchor protein fusion was found partially dissociated into dimers. The coexpression of free and anchored subunits significantly improved the display efficiency and catalytic activity. Given that oligomeric enzymes may adopt a non-native conformation on the cell surface, the subunit interfaces of surface-displayed β-glucuronidase were in situ characterized using a Förster resonance energy transfer (FRET) strategy, and the tetrameric structure was well maintained in the coexpressed β-glucuronidases. Finally, the coexpression strategy was applied to yeast-surface-displayed oligomeric cellulases, significantly enhancing the activities of tetrameric endoglucanase and dimeric β-glucosidase and the concentration of cellulosic ethanol for the two-enzyme codisplaying strain. This work provides insights into the structure-activity relationship and the efficient utilization of surface-displayed oligomeric enzymes.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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