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MUS81-MMS4 structure-specific endonuclease complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Summary
Structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the mitotic recombination intermediates. Has a substrate preference for branched DNA structures with a 5-prime end at the branch nick with cleavage probably occurring approximately half a helical turn upstream of the free 5-prime end. Resolves the four-way Holliday junction (HJ) in which the two recombining DNAs are covalently-linked junctions in crossover formation during meiotic recombination, however, its ability to cut intact HJs was found to be very limited in comparison with other structures, suggesting that a HJ precursor (such as a nicked HJ) might be its preferred DNA substrate. In mitotic cells, the activity of MUS81-MMS4 is low during S-phase, but phosphorylation of the MMS4 leads to complex activation at the onset of mitosis by the collaborative actions of two cell cycle kinases: CDC28 (P00546) and CDC5 (P32562).
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

hydrolase activity, nuclease activity, nuclear replication fork

Molecular Function

Manually curated GO annotations reflect our best understanding of the basic molecular function for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

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Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Cellular Component

Manually curated GO annotations reflect our best understanding of the basic cellular component for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Shared Annotations

This diagram displays Gene Ontology terms (green) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). Note: No diagram is shown ("No shared annotations") if there are less than 2 shared GO annotations between this complex and any other complexes.

No shared annotations