BIM1 / YER016W Overview
- Standard Name
- BIM1 1
- Systematic Name
- YER016W
- SGD ID
- SGD:S000000818
- Aliases
- EB1 3 , YEB1 6
- Feature Type
- ORF , Verified
- Description
- Microtubule plus end-tracking protein; forms a complex with Kar9p that makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1) 2 3 4 5
- Name Description
- BInding to Microtubules 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Bim1p is 344 amino acids long, low in abundance, long-lived; phosphorylated on 16 residues
- Length (a.a.)
- 344
- Mol. Weight (Da)
- 38350.0
- Isoelectric Point
- 5.05
- Median Abundance (molecules/cell)
- 2653 +/- 1031
- Half-life (hr)
- 12.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BIM1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Microtubule plus-end binding protein involved in microtubule depolymerization, microtubule nucleation, mitotic sister chromatid cohesion and mitotic spindle orientation checkpoint; also localizes to the spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables microtubule plus-end binding (IDA)
Biological Process
- Manually Curated
- involved in 2-micrometer plasmid partitioning (IGI, IMP)
- involved in microtubule depolymerization (IMP)
- acts upstream of or within microtubule nucleation (IMP)
- involved in mitotic sister chromatid cohesion (IMP, IGI)
- involved in mitotic spindle orientation checkpoint signaling (IGI)
- involved in negative regulation of microtubule depolymerization (IMP)
- involved in nuclear migration along microtubule (IMP)
- involved in positive regulation of microtubule polymerization (IDA)
- involved in protein localization to microtubule plus-end (IMP)
Cellular Component
- Manually Curated
- located in cytoplasmic microtubule (IDA)
- located in microtubule plus-end (IDA)
- located in mitotic spindle (IDA)
- located in spindle midzone (IDA)
- located in spindle pole (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays chromosome instability (CIN) and increased chronological lifespan; overexpression leads to slow growth and abnormal G2 phase progression and budding index
Classical Genetics
- overexpression
- null
- cell cycle progression in metaphase: increased duration
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- cold sensitivity: increased
- colony sectoring: increased
- heat sensitivity: increased
- nuclear fusion during mating: decreased
- nuclear position: abnormal
- protein activity: decreased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- spindle morphology: abnormal
- reduction of function
- repressible
- null
- chronological lifespan: increased
- colony sectoring: increased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- innate thermotolerance: increased
- prion formation: increased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: increased rate
- sporulation: absent
- UV resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- reduction of function
- acid pH resistance: decreased
- heat sensitivity: increased
- metal resistance: decreased
- osmotic stress resistance: decreased
- osmotic stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- starvation resistance: decreased
- starvation resistance: increased
- utilization of carbon source: decreased
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Bim1p interacts physically with proteins involved in cytoskeleton organization; BIM1 interacts genetically with genes involved in mitotic cell cycle
1352 total interactions for 570 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 18
- Biochemical Activity: 2
- Co-crystal Structure: 3
- Co-fractionation: 2
- Co-localization: 2
- Co-purification: 2
- PCA: 2
- Protein-peptide: 1
- Reconstituted Complex: 21
- Two-hybrid: 37
Genetic Interactions
- Dosage Rescue: 7
- Negative Genetic: 443
- Phenotypic Enhancement: 196
- Phenotypic Suppression: 5
- Positive Genetic: 54
- Synthetic Growth Defect: 167
- Synthetic Lethality: 344
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-04-22
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.