SPC72 / YAL047C Overview
- Standard Name
- SPC72 1
- Systematic Name
- YAL047C
- SGD ID
- SGD:S000000045
- Aliases
- LDB4 7
- Feature Type
- ORF , Verified
- Description
- Gamma-tubulin small complex (gamma-TuSC) receptor; recruits the gamma-TuSC complex to the cytoplasmic side of the SPB, connecting nuclear microtubules to the SPB; involved in astral microtubule formation, stabilization, and with Stu2p, anchoring astral MTs at the cytoplasmic face of the SPB, and regulating plus-end MT dynamics; regulated by Cdc5 kinase 2 3 4 5 6
- Name Description
- Spindle Pole Component 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SPC72 is located on the left arm of chromosome I between GEM1 outer mitochondrial membrane GTPase and BOL3 protein involved in Fe-S cluster transfer; coding sequence is 1869 nucleotides long with 8 nonsynonymous SNPs, 12 synonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 622
- Mol. Weight (Da)
- 72073.2
- Isoelectric Point
- 4.71
- Median Abundance (molecules/cell)
- 1185 +/- 440
- Half-life (hr)
- 5.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPC72 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Structural constituent of cytoskeleton; involved in microtubule nucleation,nuclear migration along microtubule, mitotic spindle elongation, mitotic spindle orientation checkpoint and mitotic sister chromatid segregation; localizes to outer plaque of spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables microtubule lateral binding (IDA)
- enables microtubule plus-end binding (IDA)
- enables protein-containing complex binding (IDA)
Biological Process
- Manually Curated
- involved in astral microtubule anchoring at mitotic spindle pole body (IMP)
- involved in astral microtubule organization (IMP)
- involved in cytoplasmic microtubule organization (IMP)
- involved in gamma-tubulin complex localization to cytoplasmic side of mitotic spindle pole body (IPI)
- involved in microtubule nucleation (IMP)
- involved in mitotic spindle orientation checkpoint signaling (IGI, IPI)
Cellular Component
- Manually Curated
- located in mitotic spindle astral microtubule end (IDA)
- located in outer plaque of mitotic spindle pole body (IDA)
- located in outer plaque of spindle pole body (IDA)
- located in spindle microtubule (IDA)
- located in spindle pole body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
- reduction of function
- conditional
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- bud morphology: abnormal
- cell size: increased
- chemical compound excretion: increased
- competitive fitness: decreased
- endocytosis: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- lipid particle morphology: abnormal
- mating response: absent
- prion formation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- toxin resistance: decreased
- transposable element transposition: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
225 total interactions for 138 unique genes
Physical Interactions
- Affinity Capture-MS: 21
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 16
- Biochemical Activity: 3
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 6
- Co-purification: 1
- FRET: 3
- PCA: 7
- Protein-peptide: 1
- Reconstituted Complex: 17
- Two-hybrid: 126
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 1
- Phenotypic Enhancement: 2
- Synthetic Growth Defect: 9
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.