PKC1 / YBL105C Overview
- Standard Name
- PKC1 1
- Systematic Name
- YBL105C
- SGD ID
- SGD:S000000201
- Aliases
- CLY7 15 , CLY15 9 , HPO2 16 , STT1 17 , CLY5 15 , RSF8 18
- Feature Type
- ORF , Verified
- Description
- Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) 2 3 4
- Name Description
- Protein Kinase C 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1151
- Mol. Weight (Da)
- 131473.5
- Isoelectric Point
- 7.11
- Median Abundance (molecules/cell)
- 2386 +/- 756
- Half-life (hr)
- 10.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PKC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein kinase C; involved in cell wall organization, actin filament organization, assembly of cytoplasmic P body assembly, pexophagy, regulation of nuclear-transcribed mRNA poly(A) tail shortening; localizes to cytoplasm, cytoskeleton, nucleus, site of polarized growth in classical studies and to plasma membrane and prospore septin filament array in large scale studies
View computational annotations
Molecular Function
- Manually Curated
- enables diacylglycerol-dependent serine/threonine kinase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cellular bud neck septin ring organization (IMP)
- involved in cellular response to starvation (IDA)
- involved in intracellular signal transduction (IMP)
- involved in pexophagy (IMP)
- involved in positive regulation of cytoplasmic mRNA processing body assembly (IMP)
- involved in regulation of fungal-type cell wall organization (IMP)
- involved in regulation of nuclear-transcribed mRNA poly(A) tail shortening (IMP)
- involved in signal transduction (IMP)
- involved in stress granule assembly (IDA)
Cellular Component
- Manually Curated
- located in cellular bud neck (HDA)
- located in cytoplasm (IDA)
- located in cytoplasmic stress granule (IDA)
- located in cytoskeleton (IDA)
- located in nucleus (IDA)
- located in plasma membrane (HDA)
- located in prospore septin filament array (HDA)
- located in site of polarized growth (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; mutant fails to utilize acetate as carbon source; null mutant accumulates higher levels of ethanol and secretes elevated levels of acetate; null mutant is sensitive to acetic acid at pH 4; in large-scale studies null mutant shows reduced competitive fitness, shorter chronological lifespan, and reduced sporulation efficiency; null mutant undergoes rapid lysis unless osmotically stabilized; null mutant was scored as inviable in a systematic study performed in S288C
Classical Genetics
- repressible
- overexpression
- null
- actin cytoskeleton morphology: abnormal
- cell size: increased
- chemical compound accumulation: decreased
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- inviable
- pexophagy: absent
- protein/peptide modification: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- conditional
- reduction of function
- activation
- null
- reduction of function
- conditional
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1664 total interactions for 1115 unique genes
Physical Interactions
- Affinity Capture-MS: 34
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 11
- Biochemical Activity: 29
- Co-localization: 1
- Co-purification: 1
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 4
- Two-hybrid: 53
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 7
- Dosage Rescue: 98
- Negative Genetic: 1079
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 30
- Positive Genetic: 225
- Synthetic Growth Defect: 8
- Synthetic Lethality: 41
- Synthetic Rescue: 23
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-03-22
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.