REB1 / YBR049C Overview
- Standard Name
- REB1 1
- Systematic Name
- YBR049C
- SGD ID
- SGD:S000000253
- Aliases
- GRF2 3 , EBF1 4
- Feature Type
- ORF , Verified
- Description
- RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription 1 2
- Name Description
- RNA polymerase I Enhancer Binding protein 1
- Paralog
- NSI1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 810
- Mol. Weight (Da)
- 91825.5
- Isoelectric Point
- 4.76
- Median Abundance (molecules/cell)
- 5582 +/- 1829
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all REB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding protein in the nucleus involved in regulation of transcription by RNA polymerase II, and in the termination of transcription by RNA polymerase I
View computational annotations
Molecular Function
- Manually Curated
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in termination of RNA polymerase II transcription (IMP)
- involved in transcriptional start site selection at RNA polymerase II promoter (IDA, IMP)
Cellular Component
- Manually Curated
- located in nucleoplasm (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in strain S288C, in which conditional mutants are heat-sensitive, and reduced function mutants are sensitive to microtubule-destabilising agent benomyl; non-essential gene in strain Sigma1278b, in which null mutants are impaired in filamentous growth
Classical Genetics
- null
- overexpression
- conditional
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The reb1 null mutant is inviable; the null mutant of paralog nsi1 is viable; the reb1 nsi1 double mutant has not been annotated for phenotype.
671 total interactions for 643 unique genes
Physical Interactions
- Affinity Capture-MS: 56
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 1
- Co-localization: 1
- Co-purification: 1
- FRET: 2
- PCA: 3
- Protein-RNA: 3
- Proximity Label-MS: 2
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 2
- Negative Genetic: 334
- Positive Genetic: 254
- Synthetic Growth Defect: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- REB1 encodes an essential myb-family transcription factor initially implicated in the regulation of ribosomal RNA transcription. The Reb1p consensus binding site (5'-CGGGTRR-3'), however, is also found in the promoters of a large number of genes transcribed by RNA Polymerase II, as well as in centromeres and in subtelomeric regions. Further studies have suggested that Reb1p may indeed function as a weak transcriptional activator of a variety of protein-coding genes throughout the genome, including ACT1, FAS1 and FAS2, CLN2. Multiple Reb1p binding sites are located within the subtelomeric regions, where they appear to act as anti-silencing barriers that prevent the spreading of silenced chromatin. More recently, Reb1p has been identified as one of several factors, including Abf1p, Rsc3p, Rap1p, Mcm1p, Tbf1p and Cep3p, whose binding to the promoter region prevents nucleosome formation in the vicinity, thus generating a nucleosome-free region around the promoter and facilitating the process of transcription initiation.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.