MEC1 / YBR136W Overview
- Standard Name
- MEC1 1
- Systematic Name
- YBR136W
- SGD ID
- SGD:S000000340
- Aliases
- ESR1 2 , SAD3 31 , RAD31 32
- Feature Type
- ORF , Verified
- Description
- Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres 1 2 3 4 5 6 7 8 9 10 11 12
- Name Description
- Mitosis Entry Checkpoint 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 2368
- Mol. Weight (Da)
- 273363.7
- Isoelectric Point
- 8.45
- Median Abundance (molecules/cell)
- 224 +/- 204
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MEC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine kinase essential for DNA replication, DNA repair and telomere maintenance; localizes to the nucleus and mitochondria
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (IGI, IDA)
Biological Process
- Manually Curated
- involved in DNA recombination (IMP)
- involved in DNA replication (IMP)
- involved in positive regulation of DNA-templated DNA replication (IMP)
- involved in reciprocal meiotic recombination (IMP)
- involved in signal transduction in response to DNA damage (IMP)
- involved in telomere maintenance (IDA)
- involved in telomere maintenance via recombination (IGI)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; in an SML1 mutant background the null mutant displays decreased resistance to UV light, ionizing radiation, and DNA replication inhibitors that are also DNA damaging agents; null mutant inhibits phosphorylation of Rad26p, Cdc13p, and Ddc1p, and increases sumoylation of Rfa1p, Rfa2p, and Rad52p; reduction-of-function mutant has decreased G1 arrest, reduced chronological lifespan and increased apoptosis following nutrient depletion; reduction-of-function mutant has elevated mitotic recombination and decreased telomere length; overexpression results in reduced vegetative growth; heterozygous diploid null mutant has elevated chromosomal instability
Classical Genetics
- null
- inviable
- ionizing radiation resistance: decreased
- meiotic recombination: absent
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- spore germination: decreased
- sporulation: absent
- UV resistance: decreased
- reduction of function
- apoptosis: increased
- budding index: increased
- cell cycle progression in G1 phase: increased
- chronological lifespan: decreased
- cold sensitivity: increased
- heat sensitivity: increased
- mitotic recombination: increased
- protein/peptide distribution: increased
- resistance to chemicals: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- telomere length: decreased
- toxin resistance: decreased
- UV resistance: absent
- UV resistance: decreased
- vegetative growth: decreased
- viability: decreased
- unspecified
- conditional
- activation
- overexpression
- unspecified
- null
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast MEC1 is homologous to human ATM and ATR, and has been used to study ataxia telangiectasia, Seckel syndrome, and carcinogenesis
Manually Curated
- ataxia telangiectasia (ISS, IMP)
- breast cancer (IMP, ISS)
- colon cancer (ISS, IMP)
- endometrial cancer (ISS, IMP)
- non-Hodgkin lymphoma (ISS, IMP)
- Seckel syndrome (ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
813 total interactions for 385 unique genes
Physical Interactions
- Affinity Capture-MS: 70
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 31
- Biochemical Activity: 43
- Co-crystal Structure: 2
- Co-localization: 2
- Protein-peptide: 1
- Reconstituted Complex: 8
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 15
- Dosage Rescue: 23
- Negative Genetic: 146
- Phenotypic Enhancement: 100
- Phenotypic Suppression: 74
- Positive Genetic: 22
- Synthetic Growth Defect: 115
- Synthetic Lethality: 41
- Synthetic Rescue: 108
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-03-24
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.