GLK1 / YCL040W Overview
- Standard Name
- GLK1 1
- Systematic Name
- YCL040W
- SGD ID
- SGD:S000000545
- Aliases
- HOR3 18
- Feature Type
- ORF , Verified
- Description
- Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication 2 3 4
- Name Description
- GLucoKinase 1
- Paralog
- EMI2 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- GLK1 is located on the left arm of chromosome III between PDI1 protein disulfide isomerase and GID7 GID complex subunit; coding sequence is 1503 nucleotides long with 15 SNPs, two of which cause amino acid polymorphisms Q/H at residue 20 and V/I at residue 190; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein is 500 amino acids long with 7 hexokinase domains; localizes to cytosol and plasma membrane; protein abundance increases under exposure to hydroxyurea; shares domains with proteins involved in transmembrane transport; subject to various post-translational modifications, including acetylation, phosphorylation, succinylation, and ubiquitinylation at 23 different residues
- Length (a.a.)
- 500
- Mol. Weight (Da)
- 55367.0
- Isoelectric Point
- 6.13
- Median Abundance (molecules/cell)
- 20699 +/- 12384
- Half-life (hr)
- 15.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GLK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Glucokinase involved in glucose import, glycolysis, and mannose metabolism; localizes to cytosol and plasma membrane
View computational annotations
Molecular Function
- Manually Curated
- enables glucokinase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in D-glucose import (IGI)
- involved in glucose metabolic process (IMP)
- involved in glycolytic process (IDA)
- involved in mannose metabolic process (IDA)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- located in plasma membrane (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has lower survival rate in stationary phase and decreased chronological lifespan
Classical Genetics
- null
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast GLK1 is homologous to human GCK, and has been used to study GCK variants associated with hyperinsulinemic hypoglycemia and hyperglycemia associated with GCK-maturity-onset diabetes of the young (GCK-MODY)
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The glk1 null mutant is viable; the null mutant of paralog emi2 is viable; the glk1 emi2 double mutant displays a synthetic growth defect.
91 total interactions for 78 unique genes
Physical Interactions
- Affinity Capture-MS: 30
- Affinity Capture-RNA: 6
- Biochemical Activity: 1
- Co-purification: 2
- PCA: 3
- Proximity Label-MS: 4
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Negative Genetic: 24
- Phenotypic Enhancement: 7
- Positive Genetic: 3
- Synthetic Growth Defect: 1
- Synthetic Lethality: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Transcription is upregulated by Znf1p, Msn2p, and Msn4p; in response to hypoxia, transcription is downregulated by Ixr1p; in response to heat, transcription is regulated by Bur6p, Med2p, Med4p, Spt3p, Spt6p, Sua7p, Ume6p, Yap1p, Yap6p and Ncb2p; in response to stress, transcription is regulated by Sfp1p
- Regulators
- 47
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-11-29
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.