SPT15 / YER148W Overview
- Standard Name
- SPT15 1 2
- Systematic Name
- YER148W
- SGD ID
- SGD:S000000950
- Aliases
- BTF1 49 , TBP , TBP1
- Feature Type
- ORF , Verified
- Description
- TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP 1 3 4
- Name Description
- SuPpressor of Ty insertions 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 240
- Mol. Weight (Da)
- 27011.6
- Isoelectric Point
- 9.93
- Median Abundance (molecules/cell)
- 4563 +/- 809
- Half-life (hr)
- 4.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPT15 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA- and chromatin-binding general transcription factor that recruits other transcription factors in preinitiation complex assembly for RNA polymerases II and III, and rDNA transcription by RNA polymerase I; subunit of TFIID and TFIIIB; also involved in the chromosomal incorporation of transposable elements
View computational annotations
Molecular Function
- Manually Curated
- enables chromatin binding (IDA)
- enables DNA binding, bending (IDA)
- enables RNA polymerase I general transcription initiation factor binding (IPI, IDA)
- enables RNA polymerase II core promoter sequence-specific DNA binding (IDA)
- enables RNA polymerase II-specific DNA-binding transcription factor binding (IDA, IPI)
- enables RNA polymerase III transcription regulatory region sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in nucleolar large rRNA transcription by RNA polymerase I (IMP)
- involved in RNA polymerase I preinitiation complex assembly (IMP)
- involved in RNA polymerase II preinitiation complex assembly (IMP)
- involved in RNA polymerase III preinitiation complex assembly (IDA)
Cellular Component
- Manually Curated
- located in nucleus (IDA)
- part of transcription factor TFIID complex (IDA)
- part of transcription factor TFIIIB complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants grow slowly, are sensitive to heat and DNA-damaging agent MMS; some mutations increase ethanol tolerance; heterozygous diploid null is haploinsufficient; overexpression interferes with silencing
Classical Genetics
- unspecified
- conditional
- reduction of function
- overexpression
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SPT15 is homologous to human TBP which, in contrast to the yeast gene, contains CAG (polyQ) repeats whose expansion has been implicated in spinocerebellar ataxia, and which are subject to inhibition by toxic huntingtin via structural destabilization
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
817 total interactions for 339 unique genes
Physical Interactions
- Affinity Capture-MS: 211
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 169
- Biochemical Activity: 3
- Co-crystal Structure: 10
- Co-fractionation: 5
- Co-localization: 27
- Co-purification: 12
- Cross-Linking-MS (XL-MS): 1
- Far Western: 1
- PCA: 3
- Protein-peptide: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 105
- Two-hybrid: 13
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 42
- Negative Genetic: 144
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 3
- Positive Genetic: 15
- Synthetic Growth Defect: 10
- Synthetic Lethality: 14
- Synthetic Rescue: 18
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SPT15 encodes TATA-binding protein (TBP), a general transcription factor of the beta-sheet class. TBP is a component of the RNA polymerase I core factor, TFIID, and TFIIIB, and is essential for positioning the appropriate RNA polymerase at the transcription start site. As a component of the polymerase I core factor, TBP regulates the transcription of rRNA. As a member of the TFIID complex, TBP is required for RNA polymerase II-mediated basal transcription of protein-coding genes and some small nuclear RNAs. As part of TFIIIB, TBP is involved in the transcription of tRNAs, most small nuclear RNAs, and the 5S rRNA. TBP binds the canonical 5'-TATAAAAG-3' sequence, primarily in the minor groove, which unwinds the helix and bends the DNA. TBP self-regulates through the formation of homodimers, which are inactive and must disassociate before the protein is able to bind DNA. Dimer dissociation and TBP/promoter association are the rate-limiting steps in the formation of transcriptional pre-initiation complexes.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-02-26
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.