LAG1 / YHL003C Overview
- Standard Name
- LAG1 1
- Systematic Name
- YHL003C
- SGD ID
- SGD:S000000995
- Feature Type
- ORF , Verified
- Description
- Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication 2 3 4 5 6
- Name Description
- Longevity Assurance Gene 1
- Paralog
- LAC1 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- LAG1 has a paralog, LAC1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 411
- Mol. Weight (Da)
- 48462.9
- Isoelectric Point
- 9.17
- Median Abundance (molecules/cell)
- 4848 +/- 2126
- Half-life (hr)
- 14.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all LAG1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sphingosine N-acyltransferase subunit of the acyl-CoA ceramide synthase complex, involved in ceramide biosynthesis and replicative cell aging
View computational annotations
Molecular Function
- Manually Curated
- enables sphingosine N-acyltransferase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in ceramide biosynthetic process (IMP, IDA)
Cellular Component
- Manually Curated
- part of acyl-CoA ceramide synthase complex (IDA)
- located in endoplasmic reticulum (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant accumulates free sphingoid long-chain bases and has abnormally aggregated mitochondria; overexpression causes slow growth
Classical Genetics
- null
- chronological lifespan: decreased
- competitive fitness: increased
- haploinsufficient
- oxidative stress resistance: increased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation: normal
- transposable element transposition: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast LAG1 is homologous to human CERS2, and has been used to study fatty liver disease, type 2 diabetes mellitus, and hepatocellular carcinoma
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The lag1 null mutant is viable; the null mutant of paralog lac1 is viable; the lag1 lac1 double mutant is inviable or displays a growth defect.
169 total interactions for 112 unique genes
Physical Interactions
- Affinity Capture-MS: 25
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 4
- Biochemical Activity: 3
- Co-localization: 1
- Co-purification: 1
- PCA: 14
- Reconstituted Complex: 1
- Two-hybrid: 7
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Rescue: 4
- Negative Genetic: 59
- Phenotypic Enhancement: 3
- Positive Genetic: 28
- Synthetic Growth Defect: 7
- Synthetic Lethality: 4
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.