CDC14 / YFR028C Overview
- Standard Name
- CDC14 1
- Systematic Name
- YFR028C
- SGD ID
- SGD:S000001924
- Aliases
- OAF3 44
- Feature Type
- ORF , Verified
- Description
- Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response, autophagy during meiosis; downregulates microautophagy induction after TORC1 inactivation; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of cdc14-1 mutant 2 3 4 5 7 8 9 10 11 12 13 14
- Name Description
- Cell Division Cycle 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cdc14p is 551 amino acids long, shorter-lived, low in abundance; sumoylated on K203, phosphorylated on 29 residues
- Length (a.a.)
- 551
- Mol. Weight (Da)
- 61909.4
- Isoelectric Point
- 8.09
- Median Abundance (molecules/cell)
- 3030 +/- 681
- Half-life (hr)
- 8.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC14 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphatase involved in mitotic exit, chromosome segregation, meiotic spindle disassembly, stress response and cytokinesis; subunit of the RENT complex; localizes to the nucleolus and spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables phosphoprotein phosphatase activity (IDA)
- enables protein serine/threonine phosphatase activity (IDA)
Biological Process
- Manually Curated
- involved in autophagy of mitochondrion (IMP)
- involved in cellular response to osmotic stress (IMP)
- involved in chromosome segregation (IMP)
- involved in meiotic nuclear division (IDA)
- involved in meiotic spindle disassembly (IMP)
- involved in mitotic cell cycle (IMP)
- involved in negative regulation of transcription by RNA polymerase I (IMP)
- involved in positive regulation of autophagosome assembly (IMP)
- involved in positive regulation of autophagy (IDA)
- involved in positive regulation of cytokinesis (IMP)
- involved in positive regulation of mitotic actomyosin contractile ring assembly (IMP, IGI)
- involved in rDNA chromatin condensation (IMP)
- involved in regulation of exit from mitosis (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in cytoplasm (IDA, IMP)
- located in mitotic spindle pole body (IDA)
- located in new mitotic spindle pole body (IDA)
- located in nucleolus (IDA)
- located in nucleus (IDA, HDA)
- part of RENT complex (IDA)
- located in spindle pole body (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutations cause cell cycle arrest in late anaphase with large-budded cells; overexpression results in slow growth
Classical Genetics
- conditional
- actin cytoskeleton morphology: abnormal
- autophagy: decreased
- cell cycle progression in late anaphase: arrested
- cell cycle progression: arrested
- cellular morphology: abnormal
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- heat sensitivity: increased
- mitophagy: decreased
- mitotic recombination: increased
- nuclear morphology: abnormal
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- sporulation: absent
- vegetative growth: decreased
- repressible
- null
- overexpression
- misexpression
- reduction of function
- null
- repressible
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CDC14 is homologous to human CDC14A and CDC14B, and has been used to study vascular disease, colorectal carcinoma, and autosomal recessive nonsyndromic deafness 32
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cdc14p interacts physically with proteins involved in mitotic cell cycle; CDC14 interacts genetically with genes involved in mitotic cell cycle
935 total interactions for 562 unique genes
Physical Interactions
- Affinity Capture-MS: 239
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 66
- Biochemical Activity: 41
- Co-crystal Structure: 6
- Co-localization: 6
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 1
- PCA: 2
- Protein-peptide: 3
- Proximity Label-MS: 1
- Reconstituted Complex: 25
- Two-hybrid: 27
Genetic Interactions
- Dosage Lethality: 8
- Dosage Rescue: 38
- Negative Genetic: 312
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 31
- Positive Genetic: 24
- Synthetic Growth Defect: 22
- Synthetic Lethality: 24
- Synthetic Rescue: 24
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-05-18
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.