PHO2 / YDL106C Overview
- Standard Name
- PHO2
- Systematic Name
- YDL106C
- SGD ID
- SGD:S000002264
- Aliases
- phoB 9 , BAS2 10 , GRF10 11
- Feature Type
- ORF , Verified
- Description
- Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia 1 2 3 4
- Name Description
- PHOsphate metabolism
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 559
- Mol. Weight (Da)
- 63362.5
- Isoelectric Point
- 4.7
- Median Abundance (molecules/cell)
- 2345 +/- 559
- Half-life (hr)
- 5.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PHO2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-binding transcription factor involved in regulation of purine nucleobase metabolism, histidine biosynthesis, and phosphate metabolism; localizes to the nucleus and also to the cytosol during response to hypoxia
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in chromatin remodeling (IMP)
- involved in positive regulation of DNA binding (IDA, IMP)
- involved in positive regulation of phosphate metabolic process (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IGI, IMP)
- involved in positive regulation of transcription regulatory region DNA binding (IMP)
- involved in regulation of histidine biosynthetic process (IGI, IMP)
- involved in regulation of purine nucleobase metabolic process (IDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- reduction of function
- null
- alkaline pH resistance: decreased
- bud morphology: abnormal
- cell cycle progression in G1 phase: delayed
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- freeze-thaw resistance: decreased
- haploproficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- nutrient utilization: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- respiratory growth: decreased
- respiratory growth: decreased rate
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- utilization of phosphorus source: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
256 total interactions for 187 unique genes
Physical Interactions
- Affinity Capture-MS: 25
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 4
- Biochemical Activity: 1
- Co-localization: 1
- Co-purification: 1
- PCA: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 14
- Two-hybrid: 11
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 160
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 6
- Positive Genetic: 15
- Synthetic Growth Defect: 5
- Synthetic Lethality: 1
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- PHO2 encodes a helix-turn-helix transcription factor of the homeodomain family that recognizes the binding motif '5-WTAWTA-3'. Pho2p activates genes involved in several different processes including purine nucleotide biosynthesis (ADE1, ADE2, ADE8 and ADE5,7), histidine biosynthesis (HIS4), tryptophan biosynthesis (TRP4), and phosphate utilization (PHO5, PHO81). Pho2p activates transcription along with one of at least three distinct partner proteins: the zinc finger protein Swi5p, the basic-helix-loop-helix factor Pho4p, and Bas1p, a myb-like activator. Pho2p and Pho4p cooperatively bind to the promoter site of the PHO5 acid phosphatase, and are required for PHO5 expression when cells are starved of phosphate. Pho2p and Swi5p together activate HO, while Pho2p and Bas1p activate genes in the purine and histidine biosynthesis pathways. Pho2p is phosphorylated by cdc2/CDC28-type kinase at the 'SPIK' site (amino acids 230-233), which facilitates its interaction with Pho4p and is required for PHO5 expression under low phosphate conditions.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2003-03-13
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.