CRF1 / YDR223W Overview
- Standard Name
- CRF1 1
- Systematic Name
- YDR223W
- SGD ID
- SGD:S000002631
- Feature Type
- ORF , Verified
- Description
- Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication 1 2 3
- Name Description
- Co-Repressor with FHL1 1
- Paralog
- IFH1 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CRF1 has a paralog, IFH1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 467
- Mol. Weight (Da)
- 51668.8
- Isoelectric Point
- 4.58
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CRF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription corepressor that negative regulates transcription of ribosomal protein genes; localizes to both cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables transcription corepressor activity (IDA)
Biological Process
- Manually Curated
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits decreased sensitivity to rapamycin and reduced repression of ribosomal protein genes in response to rapamycin treatment; overexpression causes slow growth
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The crf1 null mutant is viable; the null mutant of paralog ifh1 is inviable; the crf1 ifh1 double mutant has not been annotated for phenotype.
42 total interactions for 30 unique genes
Physical Interactions
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 1
- Biochemical Activity: 3
- PCA: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 2
- Negative Genetic: 20
- Phenotypic Suppression: 1
- Positive Genetic: 2
- Synthetic Growth Defect: 4
- Synthetic Lethality: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CRF1 encodes a transcription corepressor that regulates genes involved in growth and metabolism. The complex of Crf1p with the forkhead domain transcription factor Fhl1p represses transcription of ribosomal protein-encoding genes under conditions of nutrient deficiency. Crf1p binding to Fhl1p is mutually exclusive with binding of the Crf1p paralog Ifh1p, which acts as a transcriptional coactivator for Fhl1p. Crl1p activity is regulated via its localization to the nucleus or cytoplasm. When nutrients are abundant, TORC1 inhibits the Yak1p kinase via regulation of protein kinase A (PKA). Inhibition of Yak1p activity causes Crf1p to become dephosphorylated and exit the nucleus, relieving repression of the ribosomal protein genes and allowing Ifh1p to bind Fhl1p and activate transcription. When nutrients are scarce, or when TORC1 is inhibited with rapamycin, TORC1 does not inhibit Yak1p and Crf1p becomes phosphorylated. The phosphorylated form is able to accumulate in the nucleus, where it binds to Fhl1p and represses ribosomal protein gene transcription. Interestingly, this mechanism appears not to occur in the common lab strain background W303: the crf1 null mutation in W303 has no phenotype, and Crf1p cannot be detected at promoters. Since rapamycin treatment does repress ribosomal protein gene transcription in W303, this suggests that TORC1 regulation can occur through additional mechanisms.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.