CAD1 / YDR423C Overview


Standard Name
CAD1 1
Systematic Name
YDR423C
SGD ID
SGD:S000002831
Aliases
YAP2 4
Feature Type
ORF , Verified
Description
AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication 2 3 4 5 6 7 8 10
Name Description
CADmium resistance 9
Paralog
YAP1 10
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
CAD1 has a paralog, YAP1, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
409
Mol. Weight (Da)
45751.4
Isoelectric Point
7.09
Median Abundance (molecules/cell)
1183 +/- 688

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all CAD1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence-specific DNA-binding transcription factor involved in the cellular responses to stress and to cadmium; localizes to both the cytoplasm and nucleus

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant is sensitive to oxidative stress; heterozygous diploid null is haploinsufficient; overexpression increases cadmium resistance and filamentous growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The cad1 null mutant is viable; the null mutant of paralog yap1 is viable; the cad1 yap1 double mutant displays a growth defect.

99 total interactions for 87 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 3
  • FRET: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Lethality: 2
  • Dosage Rescue: 3
  • Negative Genetic: 46
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 7
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
CAD1 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and binds to a consensus cis-acting Yap recognition element (YRE; 5'-TTAC/GTAA-3'). Cad1p is a key player in the cellular response to cadmium and other toxic compounds. The leader sequence of the CAD1 mRNA contains two upstream open reading frames (uORF) that act to block ribosomal scanning and are involved in mRNA turnover via termination-dependent decay. The activity of Cad1p is regulated at the level of its export from the nucleus. Under normal conditions Cad1p is freely distributed between the cytoplasm and nucleus, but mostly it is localized in the cytosol due to its active export from the nucleus by Crm1p, which binds a nuclear export signal (NES) in the C-terminal cysteine-rich domain (CRD) of Cad1p. Cad1p is activated by cadmium, which binds the c-CRD, abolishing the recognition of the NES by Crm1p. The only Cad1p target gene identified thus far is FRM2, a type II nitroreductase involved in the cellular response to oxidative stress and the regulation of fatty acid metabolism. Cad1p also binds the Rck1p protein kinase under conditions of oxidative stress.
Regulators
1
Targets
253
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
28
Additional
48
Reviews
17

Resources