DOT1 / YDR440W Overview
- Standard Name
- DOT1 1
- Systematic Name
- YDR440W
- SGD ID
- SGD:S000002848
- Aliases
- KMT4 7 , PCH1 3
- Feature Type
- ORF , Verified
- Description
- Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response; single DOT1 gene produces two Dot1p isoforms from alternative translation start sites as a result of leaky scanning by the ribosome 2 3 4 5 6
- Name Description
- Disruptor Of Telomeric silencing 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- DOT1/YDR440W is located on the right arm of chromosome IV toward the telomere between LRS4 nucleolar protein and APT2 adenine phosphoribosyltransferase; coding sequence is 1749 nucleotides long with 15 SNPs, 7 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Dot1p is 582 amino acids long, somewhat short-lived, low in abundance; contains disordered regions in N-terminus; ubiquitinylated on K43 and K50, acetylated on 10 residues, phosphorylated on 15 residues
- Length (a.a.)
- 582
- Mol. Weight (Da)
- 66219.2
- Isoelectric Point
- 9.46
- Median Abundance (molecules/cell)
- 1310 +/- 850
- Half-life (hr)
- 6.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DOT1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Histone methyltransferase, specific to H3-K79; involved in DNA damage checkpoint, recombinational and nucleotide-excision repair and chromatin silencing at telomeres; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables histone H3K79 methyltransferase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in DNA damage checkpoint signaling (IMP, IGI)
- involved in global genome nucleotide-excision repair (IMP)
- involved in meiotic recombination checkpoint signaling (IGI)
- involved in mitotic G1 DNA damage checkpoint signaling (IMP)
- involved in mitotic intra-S DNA damage checkpoint signaling (IMP)
- involved in negative regulation of heterochromatin formation (IMP)
- involved in nucleosome assembly (IDA)
- involved in nucleotide-excision repair (IGI, IMP)
- involved in postreplication repair (IGI)
- involved in recombinational repair (IGI, IMP)
- involved in subtelomeric heterochromatin formation (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits sensitivity to gamma rays but resistance to X rays and MMS; null mutant shows decreased silencing and has telomeres of heterogeneous length; overexpression also causes decreased silencing; in large-scale studies, null mutant has shortened chronological lifespan, increased resistance to heat, and altered resistance to various chemicals
Classical Genetics
- null
- chemical compound accumulation: increased
- gamma ray resistance: decreased
- mutation frequency: increased
- protein/peptide distribution: increased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- silencing: decreased
- telomere length: abnormal
- X ray resistance: increased
- overexpression
- null
- chitin deposition: decreased
- chronological lifespan: decreased
- haploinsufficient
- heat sensitivity: increased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- resistance to enzymatic treatment: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Dot1p interacts physically with proteins involved in chromatin organization and DNA repair; DOT1 interacts genetically with genes involved in DNA repair and transcription
469 total interactions for 313 unique genes
Physical Interactions
- Affinity Capture-MS: 8
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 6
- Biochemical Activity: 18
- Co-crystal Structure: 3
- Co-localization: 3
- Far Western: 1
- Reconstituted Complex: 8
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Rescue: 2
- Negative Genetic: 275
- Phenotypic Enhancement: 23
- Phenotypic Suppression: 20
- Positive Genetic: 13
- Synthetic Growth Defect: 42
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 6
- Synthetic Rescue: 28
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DOT1 promoter is bound by Cin5p in response to heat; DOT1 transcription is regulated by Pdr1p
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-08-19
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.