PHO8 / YDR481C Overview
- Standard Name
- PHO8 1
- Systematic Name
- YDR481C
- SGD ID
- SGD:S000002889
- Aliases
- phoH 6
- Feature Type
- ORF , Verified
- Description
- Repressible vacuolar alkaline phosphatase; controls polyphosphate content; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN 2 3 4 5
- Name Description
- PHOsphate metabolism 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 566
- Mol. Weight (Da)
- 62976.5
- Isoelectric Point
- 5.33
- Median Abundance (molecules/cell)
- 3505 +/- 1618
- Half-life (hr)
- 8.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PHO8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Alkaline phosphatase involved in protein dephosphorylation and nucleotide catabolism; localizes to the vacuole membrane
View computational annotations
Molecular Function
- Manually Curated
- enables alkaline phosphatase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in nicotinamide nucleotide metabolic process (IMP)
- acts upstream of or within nucleotide catabolic process (IMP)
Cellular Component
- Manually Curated
- located in fungal-type vacuole membrane (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant accumulates reduced levels of N-ribosylnicotinamide; in large-scale studies, null mutant displays reductions in competitive fitness and starvation resistance, and is sensitive to sodium selenide
Classical Genetics
- null
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PHO8 is homologous to human ALPL, ALPP, and ALPG, and has been used to study numerous diseases including osteomalacia, rickets, cleidocranial dysplasia, infantile hypophosphatasia, calcification, primary biliary cirrhosis, cryptorchidism, and various cancers including meningioma, seminoma, germinoma, osteosarcoma, gonadoblastoma, embryonal carcinoma, and choriocarcinoma
Manually Curated
- bone disease (ISS)
- calcinosis (ISS)
- carcinoma (ISS)
- choriocarcinoma (ISS)
- cleidocranial dysplasia (ISS)
- cryptorchidism (ISS)
- embryonal carcinoma (ISS)
- germ cell cancer (ISS)
- germinoma (ISS)
- gonadoblastoma (ISS)
- hypophosphatasia (ISS)
- infantile hypophosphatasia (ISS)
- meningioma (ISS)
- osteomalacia (ISS)
- osteosarcoma (ISS)
- primary biliary cholangitis (ISS)
- rickets (ISS)
- seminoma (ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
120 total interactions for 117 unique genes
Physical Interactions
- Affinity Capture-MS: 60
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 5
- Co-fractionation: 3
- Co-localization: 1
- Protein-RNA: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Negative Genetic: 33
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 2
- Positive Genetic: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 10
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.