VPS3 / YDR495C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

VPS3/YDR495C is a non-essential gene; null mutants are viable, slow-growing, and associated with several mutant phenotypes that significantly affect cellular functions and overall fitness. In null mutants, biofilm formation is decreased, chronological lifespan is reduced, and competitive fitness is diminished. The morphology of the endomembrane system is abnormal, suggesting disruptions in intracellular organization and transport processes. Furthermore, these mutants show increased sensitivity to heat and decreased resistance to hyperosmotic stress and metal exposure, highlighting their vulnerability to various environmental challenges. Oxidative stress resistance is also reduced, which may compromise cellular integrity under stress conditions. While replicative lifespan is increased, sporulation is decreased, indicating potential trade-offs in reproductive success. Telomere length is diminished, which could affect cellular aging and stability, and vacuolar morphology is abnormal, potentially disrupting cellular storage and waste management. In cases of reduced function of VPS3, vacuolar morphology and transport are both abnormal, further emphasizing the gene's role in maintaining vacuolar integrity and function. When VPS3 is overexpressed, vacuolar morphology remains abnormal, suggesting that excessive levels of the gene may also lead to disruptions in vacuolar structure.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources