SRS2 / YJL092W Overview
- Standard Name
- SRS2 1
- Systematic Name
- YJL092W
- SGD ID
- SGD:S000003628
- Aliases
- HPR5 15 , RADH 15 , RADH1
- Feature Type
- ORF , Verified
- Description
- DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1 2 3 4 5 6 7 8 9 10 11 12 13 14
- Name Description
- Suppressor of Rad Six 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1174
- Mol. Weight (Da)
- 134321.7
- Isoelectric Point
- 9.21
- Median Abundance (molecules/cell)
- 673 +/- 579
- Half-life (hr)
- 5.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SRS2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP-dependent 3' to 5' DNA helicase involved in double-strand break repair, DNA duplex unwinding and disassembling Rad51 nucleoprotein filaments during meiosis; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables 3'-5' DNA helicase activity (IDA)
- enables enzyme activator activity (IDA)
- enables protein binding (IPI)
Biological Process
- Manually Curated
- involved in DNA duplex unwinding (IDA)
- involved in DNA protection (IMP)
- involved in DNA recombinase disassembly (IDA, IMP)
- involved in DNA repair (IGI)
- involved in double-strand break repair via nonhomologous end joining (IDA)
- involved in meiosis I (IMP)
- involved in negative regulation of DNA recombination (IMP)
- involved in negative regulation of double-strand break repair via homologous recombination (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows chromosome instability, increased mitotic recombination and UV sensitivity; in systematic studies null mutants are sensitive to DNA damaging factors, such as gamma rays, X rays, camptothecin, cisplating, MMS, hydroxyurea; homozygous null mutant diploids show reduced spore germination; overexpression causes delay in S phase of the cell cycle
Classical Genetics
- null
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- gamma ray resistance: decreased
- ionizing radiation resistance: decreased
- meiotic recombination: delayed
- mitotic recombination: increased
- protein/peptide distribution: abnormal
- radiation resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- spore germination: decreased
- UV resistance: decreased
- viable
- overexpression
- reduction of function
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- haploinsufficient
- heat sensitivity: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- transposable element transposition: increased
- UV resistance: decreased
- viable
- X ray resistance: decreased
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1443 total interactions for 603 unique genes
Physical Interactions
- Affinity Capture-MS: 42
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 14
- Biochemical Activity: 4
- Co-crystal Structure: 2
- PCA: 1
- Protein-peptide: 1
- Reconstituted Complex: 33
- Two-hybrid: 109
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 331
- Dosage Rescue: 43
- Negative Genetic: 268
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 53
- Positive Genetic: 41
- Synthetic Growth Defect: 193
- Synthetic Lethality: 131
- Synthetic Rescue: 139
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-12
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.