TPK1 / YJL164C Overview
- Standard Name
- TPK1 1
- Systematic Name
- YJL164C
- SGD ID
- SGD:S000003700
- Aliases
- SRA3 40 , PKA1
- Feature Type
- ORF , Verified
- Description
- cAMP-dependent protein kinase catalytic subunit; role in nutrient-stimulated growth via Ras-cAMP signaling; inhibited by regulatory subunit Bcy1p in cAMPs absence; phosphorylates and inhibits Whi3p to promote G1/S phase passage; phosphorylates Nej1p to regulate nonhomologous end joining (NHEJ); impairs mitochondrial import of pre-Tom40p via phosphorylation under non-respiratory conditions; partially redundant with Tpk2p and Tpk3p; human homolog PRKACB regulates NHEJ via NHEJ1 phosphorylation 2 3 4 6 7 8
- Name Description
- Takashi's Protein Kinase 5
- Paralog
- TPK3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TPK1 has a paralog, TPK3, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 397
- Mol. Weight (Da)
- 46064.1
- Isoelectric Point
- 5.14
- Median Abundance (molecules/cell)
- 3555 +/- 1687
- Half-life (hr)
- 9.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TPK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- cAMP-dependent protein kinase involved in signaling; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables cAMP-dependent protein kinase activity (IDA, IGI, IMP)
- enables protein kinase activity (HDA)
Biological Process
- Manually Curated
- involved in double-strand break repair via nonhomologous end joining (IMP, IGI)
- involved in negative regulation of cytoplasmic mRNA processing body assembly (IGI)
- involved in peptidyl-serine phosphorylation (IDA, IMP)
- involved in protein import into nucleus (IMP)
- involved in protein kinase A signaling (IGI)
- involved in Ras protein signal transduction (IGI, IMP)
- involved in regulation of macroautophagy (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation lowers competitive fitness and increases senitivity to desiccation; overexpression leads to abnormalities in actin cytoskeleton and blocks autophagy
Classical Genetics
- overexpression
- null
- cell death: absent
- chemical compound accumulation: decreased
- desiccation resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: absent
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- viability: decreased
- null
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The tpk1 null mutant is viable; the null mutant of paralog tpk3 is viable; the tpk1 tpk3 double mutant is viable; the tpk1 tpk2 tpk3 triple mutant is inviable.
667 total interactions for 484 unique genes
Physical Interactions
- Affinity Capture-MS: 50
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 16
- Biochemical Activity: 300
- Co-localization: 1
- PCA: 9
- Proximity Label-MS: 1
- Reconstituted Complex: 14
- Two-hybrid: 11
Genetic Interactions
- Dosage Growth Defect: 9
- Dosage Lethality: 2
- Dosage Rescue: 33
- Negative Genetic: 121
- Phenotypic Enhancement: 9
- Phenotypic Suppression: 19
- Positive Genetic: 13
- Synthetic Growth Defect: 36
- Synthetic Lethality: 8
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-05-17
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.