FPS1 / YLL043W Overview
- Standard Name
- FPS1 1
- Systematic Name
- YLL043W
- SGD ID
- SGD:S000003966
- Feature Type
- ORF , Verified
- Description
- Aquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in transport of acetate, arsenite, and antimonite; key factor in maintaining redox balance by mediating passive diffusion of glycerol; phosphorylated by Hog1p and Slt2p MAPK; deletion improves xylose fermentation; regulated by Rgc1p and Ask10p, which are regulated by Hog1p phosphorylation under osmotic stress; phosphorylation by Ypk1p required to maintain an open state 2 3 4 5 6 7 8 9 10 11
- Name Description
- FdP1 Suppressor 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Fps1p is 669 amino acids long, very short-lived, and low in abundance; ubiquitinylated on 4 lysines, phosphorylated on 31 residues
- Length (a.a.)
- 669
- Mol. Weight (Da)
- 73873.3
- Isoelectric Point
- 6.9
- Median Abundance (molecules/cell)
- 2264 +/- 862
- Half-life (hr)
- 2.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FPS1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Glycerol channel involved in transport of glycerol, arsenite, acetate, and polyols; also involved in mating and the response to arsenic; localizes to the plasma membrane
View computational annotations
Molecular Function
- Manually Curated
- enables glycerol channel activity (IMP)
- enables glycerol transmembrane transporter activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in acetate transport (IMP)
- involved in arsenite transport (IMP)
- involved in conjugation with cellular fusion (IMP)
- involved in glycerol transmembrane transport (IDA, IMP)
- involved in polyol transmembrane transport (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in cytoplasm (HDA)
- located in fungal-type vacuole (HDA)
- located in plasma membrane (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant grows slowly, is sensitive to UV, heat, starvation, formamide, Congo Red, formic acid, propanol, ethanol, Calcofluor White, DMSO, rapamycin, hydrogen peroxide; null cannot use urea as nitrogen source, has decreased replicative lifespan, increased resistance to arsenic, decreased resistance to cadmium; overexpression slows growth
Classical Genetics
- null
- cell wall morphology: abnormal
- chemical compound accumulation: increased
- fermentative metabolism: increased rate
- hydrostatic pressure resistance: decreased
- hypoosmotic stress resistance: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- metal resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- vegetative growth: decreased rate
- overexpression
- gain of function
- reduction of function
- null
- auxotrophy
- chemical compound accumulation: increased
- chitin deposition: normal
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- heat sensitivity: increased
- metal resistance: decreased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: increased rate
- starvation resistance: decreased
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Fps1p interacts physically with proteins involved in organelle assembly and cell wall organization; FPS1 interacts genetically with genes involved in mitotic cell cycle
572 total interactions for 380 unique genes
Physical Interactions
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 9
- Biochemical Activity: 11
- Co-localization: 1
- PCA: 12
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 16
- Negative Genetic: 408
- Phenotypic Enhancement: 12
- Phenotypic Suppression: 7
- Positive Genetic: 33
- Synthetic Growth Defect: 26
- Synthetic Lethality: 8
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.