HAP1 / YLR256W Overview
- Standard Name
- HAP1 1 2
- Systematic Name
- YLR256W
- SGD ID
- SGD:S000004246
- Aliases
- CYP1 8
- Feature Type
- ORF , Verified
- Description
- Zinc finger transcription factor; involved in the complex regulation of gene expression in response to levels of heme and oxygen; localizes to the mitochondrion as well as to the nucleus; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus 1 3 4 5 6 7
- Name Description
- Heme Activator Protein 1 2
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- The S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1502
- Mol. Weight (Da)
- 166107.3
- Isoelectric Point
- 7.4
- Median Abundance (molecules/cell)
- 1450 +/- 450
- Half-life (hr)
- 2.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HAP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- A transcription factor that binds to sequence-specific DNA proximal to RNA polymerase II core promoter that is involved in regulation of transcription in response to hypoxia; localized to both the nucleus and mitochondrion
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to hypoxia (IMP)
- involved in establishment of protein localization to chromatin (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of cellular respiration (IGI, IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- reduction of function
- overexpression
- null
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
79 total interactions for 74 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 4
- Biochemical Activity: 1
- PCA: 2
- Reconstituted Complex: 4
- Two-hybrid: 2
Genetic Interactions
- Negative Genetic: 32
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 4
- Synthetic Lethality: 2
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- HAP1 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Hap1p is involved in regulating gene expression in response to levels of heme and oxygen, using its ability to bind heme as a proxy for detecting oxygen levels. In the absence of heme, during hypoxic growth, Hap1p binds to the promoters of a subset of genes, such as ROX1, SOD1, HMG1, and ERG11, and acts as a transcriptional repressor to promote anaerobic growth. In the presence of heme Hap1p binds to the promoters of a different set of genes, including CYC1, CYC7, CYT1, and CTT1, which are required for aerobic growth, and upregulates their transcription. Hap1p functions as a dimer within the larger context of a complex of chaperone proteins that includes the SSA HSP70 proteins, Hsp82p, Ydj1, and Sro9p. Hap1p binding of heme causes the complex to undergo a conformational change which allows it to act as either a repressor or an activator depending on the condition. Unlike other transcription factors in the Gal4 family, Hap1p binds to asymmetric DNA sites containing a direct repeat of two CGG triplets.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.