CUE1 / YMR264W Overview
- Standard Name
- CUE1 1
- Systematic Name
- YMR264W
- SGD ID
- SGD:S000004877
- Aliases
- KIS4 5
- Feature Type
- ORF , Verified
- Description
- Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation 1 2 3 4
- Name Description
- Coupling of Ubiquitin conjugation to ER degradation 1
- Paralog
- CUE4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 203
- Mol. Weight (Da)
- 22760.9
- Isoelectric Point
- 6.69
- Median Abundance (molecules/cell)
- 3719 +/- 2487
- Half-life (hr)
- 8.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CUE1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-protein transferase activator involved in ubiquitin-dependent protein catabolic processes associated with the endoplasmic reticulum (ER) and localization of proteins to the ER membrane; an integral component of the ER membrane and associated with both Doa10p and Hrd1p ubiquitin ligase complexes
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin binding (IDA)
- enables ubiquitin-protein transferase activator activity (IDA)
Biological Process
- Manually Curated
- involved in ERAD pathway (IMP)
- involved in establishment of protein localization to endoplasmic reticulum membrane (IMP)
Cellular Component
- Manually Curated
- part of Doa10p ubiquitin ligase complex (IDA)
- located in endoplasmic reticulum (HDA)
- located in endoplasmic reticulum membrane (IDA)
- part of Hrd1p ubiquitin ligase ERAD-L complex (IDA)
- located in mitochondrion (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation causes elevated accumulation of reporter proteins and confers increased sensitivity to nickel ions; in systematic studies mutants show increased sensitivity to camptothecin MMS, amitrole and Congo Red
Classical Genetics
- null
- chemical compound accumulation: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- heat sensitivity: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: increased
- starvation resistance: decreased
- toxin resistance: decreased
- vegetative growth: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The cue1 null mutant is viable; the null mutant of paralog cue4 is viable; the cue1 cue4 double mutant has not been annotated for phenotype.
310 total interactions for 191 unique genes
Physical Interactions
- Affinity Capture-MS: 23
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 10
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-purification: 3
- PCA: 14
- Reconstituted Complex: 10
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 5
- Negative Genetic: 119
- Phenotypic Enhancement: 19
- Phenotypic Suppression: 27
- Positive Genetic: 45
- Synthetic Growth Defect: 13
- Synthetic Lethality: 3
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.