ASI3 / YNL008C Overview
- Standard Name
- ASI3 1
- Systematic Name
- YNL008C
- SGD ID
- SGD:S000004953
- Feature Type
- ORF , Verified
- Description
- Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids 1 2 3 4 5
- Name Description
- Amino acid Sensor-Independent 1
- Paralog
- ASI1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 676
- Mol. Weight (Da)
- 77643.9
- Isoelectric Point
- 6.71
- Median Abundance (molecules/cell)
- 289 +/- 181
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ASI3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-protein ligase subunit of Asi complex located in inner nuclear membrane; involved in protein catabolism and response to amino acids
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin-protein transferase activity (IGI)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cellular response to amino acid stimulus (IMP)
- involved in proteasome-mediated ubiquitin-dependent protein catabolic process (IMP)
- involved in ubiquitin-dependent protein catabolic process (IMP, IGI)
Cellular Component
- Manually Curated
- part of Asi complex (IDA)
- located in nuclear inner membrane (IDA)
- located in nuclear periphery (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; overexpression causes an increase in chromosome instability; in systematic studies null mutation confers resistance to selenomethionine and tirapazamine, increased competitive fitness, and shortened chronological lifespan
Classical Genetics
- overexpression
- null
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The asi3 null mutant is viable; the null mutant of paralog asi1 is viable; the asi3 asi1 double mutant has not been annotated for phenotype.
156 total interactions for 125 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 6
- PCA: 6
- Reconstituted Complex: 2
Genetic Interactions
- Negative Genetic: 97
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 1
- Positive Genetic: 17
- Synthetic Lethality: 2
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.