STB1 / YNL309W Overview
- Standard Name
- STB1 1 2
- Systematic Name
- YNL309W
- SGD ID
- SGD:S000005253
- Feature Type
- ORF , Verified
- Description
- Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication 3 4 5 6
- Name Description
- Sin Three Binding protein 2
- Paralog
- YOL131W 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- STB1 has a paralog, YOL131W, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 420
- Mol. Weight (Da)
- 45693.4
- Isoelectric Point
- 10.1
- Median Abundance (molecules/cell)
- 1515 +/- 1090
- Half-life (hr)
- 5.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all STB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription coactivator involved in positive regulation of transcription by RNA polymerase II; subunit of MBF transcription complex; localizes to cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coactivator activity (IPI)
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IMP, IGI)
- involved in positive regulation of mating type switching (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IGI)
- involved in regulation of transcription by RNA polymerase II (IGI, IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- part of MBF transcription complex (IDA)
- located in nucleus (HDA)
- part of SBF transcription complex (IDA)
- part of Sin3-type complex (IDA, IPI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The stb1 null mutant is viable; the null mutant of paralog yol131w is viable; the stb1 yol131w double mutant has not been annotated for phenotype.
168 total interactions for 114 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 3
- Biochemical Activity: 8
- Co-purification: 1
- PCA: 2
- Protein-RNA: 1
- Reconstituted Complex: 3
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 114
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 4
- Positive Genetic: 6
- Synthetic Growth Defect: 7
- Synthetic Lethality: 2
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- STB1 encodes a protein that contributes to the regulation of SBF and MBF target genes. SBF is a heterodimeric DNA-binding factor composed of Swi4p and Swi6p that participates in a positive feedback loop that important for producing the burst of cyclins that accompanies START. MBF is a heterodimeric DNA-binding factor composed of Mbp1p and Swi6p that promotes expression of other G1/S genes. Stb1p interacts with SBF and MBF, and binds SBF- and MBF-specific promoters during G1 phase. Stb1p functions in both transcriptional repression and activation. Stb1p serves as an intermediary between the SBF transcription complex and the Rpd3L histone deacetylase complex, and allows SBF to recruit Rpd3L to promoters. Stb1p inhibits SBF activity during early G1, until it becomes phosphorylated by CDK which ends the inhibition and allows SBF to activate transcription. Stb1p is also required binding of for Rpd3L to MBF-dependent genes.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.