DAL82 / YNL314W Overview
- Standard Name
- DAL82
- Systematic Name
- YNL314W
- SGD ID
- SGD:S000005258
- Aliases
- DURM 1
- Feature Type
- ORF , Verified
- Description
- Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain 1 2
- Name Description
- Degradation of Allantoin
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 255
- Mol. Weight (Da)
- 29079.3
- Isoelectric Point
- 7.76
- Median Abundance (molecules/cell)
- 1148 +/- 1093
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DAL82 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- A sequence-specific DNA binding transcription factor involved in both allantoin breakdown and nitrogen catabolite activation of transcription from RNA polymerase II promoter; localized to nucleus
Molecular Function
- Manually Curated
- enables sequence-specific DNA binding (IMP, IDA)
Biological Process
- Manually Curated
- involved in allantoin catabolic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IPI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; reduction of function decreases utilization of urea s nitrogen source; in systematic studies null mutants show reduced competitive fitness
Classical Genetics
- reduction of function
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
132 total interactions for 128 unique genes
Physical Interactions
- Affinity Capture-MS: 17
- Affinity Capture-RNA: 4
- Biochemical Activity: 3
- PCA: 1
- Two-hybrid: 7
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 83
- Phenotypic Enhancement: 1
- Positive Genetic: 13
- Synthetic Growth Defect: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DAL82 encodes a transcription factor that is not a member of any recognized structural class. It is smaller than most other transcription factors, and contains an N-terminal DNA-binding domain, a central domain required for transcriptional activation, and a C-terminal coiled-coil domain. In the presence of allophanate, which is the last intermediate in the allantoin degradation pathway, Dal82p activates transcription of genes encoding proteins involved in allantoin utilization, such as DAL2, DAL4, DAL5, DAL7, DUR1,2, and DUR3, and also CAR2, encoding ornithine aminotransferase involved in arginine utilization. Dal82p is one of several transcription factors involved in nitrogen source utilization (including Uga3p, Dal82p, Stp1p, and Stp2p) whose function depends on cooperation with the transcription factor Dal81p. The involvement of Dal81p in pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Dal82p binds to the UIS-ALL element in the promoters of target genes. Dal82p is able to bind DNA independently of Dal81p, but both Dal81p and Dal82p are required for transcriptional activation. The two transcription factors physically interact, and the DNA-binding domain of Dal81p is not necessary for transcriptional activation in cooperation with Dal82p.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.