SPT20 / YOL148C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

SPT20/YOL148C is a non-essential gene; null mutants are slow-growing and associated with a range of mutant phenotypes that significantly impact cellular functions and overall fitness. In null mutants, resistance to both acidic and alkaline pH is decreased, indicating a compromised ability to thrive in varying environmental conditions. These mutants exhibit abnormal bud morphology and increased sensitivity to cold, which can hinder their growth and reproduction. Competitive fitness is diminished, and the morphology of the cytoskeleton is abnormal, suggesting disruptions in cellular structure and organization. These mutants show decreased resistance to desiccation, heat, hyperosmotic stress, and oxidative stress, highlighting their vulnerability to various environmental challenges. Endocytosis is also impaired, and the ability to withstand freeze-thaw cycles is reduced. While mitophagy is increased, indicating a potential compensatory response to stress, the overall replicative lifespan is decreased, and respiratory growth is absent, suggesting severe metabolic limitations. Sporulation is also absent, which may affect reproductive success. The transposition of transposable elements is diminished, indicating changes in genomic stability. Null mutants also show increased resistance to X-ray exposure. In cases of overexpression, silencing mechanisms are abnormal.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources