PUS1 / YPL212C Overview


Standard Name
PUS1 1
Systematic Name
YPL212C
SGD ID
SGD:S000006133
Feature Type
ORF , Verified
Description
tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication 2 3 4 5 6
Name Description
PseudoUridine Synthase 1
Paralog
PUS2 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PUS1 is located on the left arm of chromosome XVI between LEA1 U2 snRNP subunit and NIP7 nucleolar protein; coding sequence is 1635 nucleotides long with 9 SNPs, 3 of which cause amino acid polymorphisms; PUS1 has paralog PUS2 from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pus1p is 544 amino acids long, of average half-life, low-to-moderate in abundance; acetylated on K442, ubiquitinylated on 3 lysines, phosphorylated on 8 residues
Length (a.a.)
544
Mol. Weight (Da)
62146.2
Isoelectric Point
8.37
Median Abundance (molecules/cell)
7833 +/- 2504
Half-life (hr)
9.2

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all PUS1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Nuclear pseudouridine synthase involved in the intramolecular conversion of uridine to pseudouridine in mRNAs, tRNAs, and snRNAs

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pus1p interacts physically with proteins involved in RNA catabolism; PUS1 interacts genetically with genes involved in transcription and tRNA processing; the pus1 null mutant is viable, the null mutant of paralog pus2 is viable, the pus1 pus2 double mutant displays a synthetic growth defect

150 total interactions for 115 unique genes

Physical Interactions

  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 7
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 2
  • Proximity Label-MS: 1

Genetic Interactions

  • Dosage Lethality: 1
  • Negative Genetic: 75
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 20
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
32
Additional
21
Reviews
13

Resources