VIK1 / YPL253C Overview
- Standard Name
- VIK1 1
- Systematic Name
- YPL253C
- SGD ID
- SGD:S000006174
- Feature Type
- ORF , Verified
- Description
- Protein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication 1 2 3 4
- Name Description
- Vegetative Interaction with Kar3p 1
- Paralog
- CIK1 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- VIK1 has a paralog, CIK1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 647
- Mol. Weight (Da)
- 75732.0
- Isoelectric Point
- 7.92
- Median Abundance (molecules/cell)
- 693 +/- 685
- Half-life (min)
- 56.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all VIK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Microtubule-binding subunit of kinesin complex involved in mitotic sister chromatid cohesion; localizes to spindle pole body
View computational annotations
Molecular Function
- Manually Curated
- enables microtubule binding (IDA)
Biological Process
- Manually Curated
- acts upstream of or within microtubule nucleation (IMP)
- involved in microtubule-based process (IMP, IPI)
- involved in mitotic sister chromatid cohesion (IMP, IGI)
Cellular Component
- Manually Curated
- part of kinesin complex (IDA)
- located in spindle pole body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene, though heterozygous diploid nulls are haploinsufficient; deletion reduces competitive fitness, decreases spore viability, and increases resistance to benomyl, cycloheximide, fenpropimorph, and bleomycin; deletion decreases resistance to camptothecin and amitrole; homozygous diploid nulls have increased resistance to methyl methanesulfonate, decreases resistance to zinc deficiency; overexpression increases genome instability and confers sensitivity to hydroxyurea
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- lipid particle morphology: abnormal
- protein secretion: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The vik1 null mutant is viable; the null mutant of paralog cik1 is viable; the vik1 cik1 double mutant is viable.
200 total interactions for 116 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 1
- Co-crystal Structure: 4
- Co-localization: 2
- Co-purification: 1
- Reconstituted Complex: 4
- Two-hybrid: 2
Genetic Interactions
- Negative Genetic: 149
- Phenotypic Enhancement: 1
- Positive Genetic: 4
- Synthetic Growth Defect: 13
- Synthetic Lethality: 9
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.