EAF3 / YPR023C Overview
- Standard Name
- EAF3 1
- Systematic Name
- YPR023C
- SGD ID
- SGD:S000006227
- Feature Type
- ORF , Verified
- Description
- Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition 1 2 3
- Name Description
- Esa1p-Associated Factor 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 401
- Mol. Weight (Da)
- 45208.0
- Isoelectric Point
- 8.51
- Median Abundance (molecules/cell)
- 2442 +/- 581
- Half-life (hr)
- 6.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all EAF3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Methylated histone binding protein involved in DNA repair, histone acetylation and deacetylation as well as regulation of RNA stability; subunit of NuA4 histone acetyltransferase complex
View computational annotations
Molecular Function
- Manually Curated
- enables methylated histone binding (IDA)
Biological Process
- Manually Curated
- involved in DNA repair (IDA)
- involved in negative regulation of antisense RNA transcription (IMP)
- involved in negative regulation of DNA-templated transcription (IMP)
- involved in regulation of DNA-templated DNA replication initiation (IMP)
- involved in regulation of RNA stability (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in transcription elongation by RNA polymerase II (IGI)
Cellular Component
- Manually Curated
- part of histone acetyltransferase complex (IDA)
- part of NuA4 histone acetyltransferase complex (IDA)
- part of Rpd3S complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased biofilm formation, increased lifespan, increased mutation frequency, abnormal utilization of dipeptides, increased protein accumulation, abnormal amino acid profiles, increased excretion of inositol, and increased resistance to acetic and lactic acids, the antifungals cycloheximide and DAPG, the DNA-damaging agents camptothecin and CG-1521, and the pesticide dieldrin ; null mutant shows decreased competitive fitness on minimal, complete, and oak exudate media, though increased fitness on gylcerol medium, decreased filamentous growth, decreased resistance to light stress, decreased utilization of glutamate as a nitrogen source, decreased transposition, and decreased resistance to caffeine, ethanol, rapamycin, MMS, DMSO, hydroxyurea, 6-azauracil, the cancer drug carboplatin, and the fungicide miconazole; heterozygous diploid is haploinsufficient; homozygous diploid null mutant shows increased heat sensitivity and increased resisitance to the pesticide dieldrin; overexpression leads to decreased vegetative growth
Classical Genetics
- null
- biofilm formation: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chemical compound excretion: increased
- competitive fitness: decreased
- competitive fitness: increased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: increased
- invasive growth: absent
- lifespan: increased
- metal resistance: decreased
- mutation frequency: increased
- nutrient utilization: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: increased
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1001 total interactions for 494 unique genes
Physical Interactions
- Affinity Capture-MS: 131
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 23
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 6
- Protein-peptide: 5
- Reconstituted Complex: 10
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 6
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 513
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 5
- Positive Genetic: 89
- Synthetic Growth Defect: 174
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 6
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.