BET2 / YPR176C Overview
- Standard Name
- BET2 1
- Systematic Name
- YPR176C
- SGD ID
- SGD:S000006380
- Feature Type
- ORF , Verified
- Description
- Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p; human homolog RABGGTB can complement yeast BET2 mutant 2 3 4
- Name Description
- Blocked Early in Transport 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 325
- Mol. Weight (Da)
- 36653.2
- Isoelectric Point
- 4.81
- Median Abundance (molecules/cell)
- 1520 +/- 1037
- Half-life (hr)
- 22.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BET2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of Rab-protein geranylgeranyltransferase complex which attaches geranylgeranyl moiety to proteins; required for vesicle-mediated transport between ER and Golgi; the complex localizes in cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables Rab geranylgeranyltransferase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in endoplasmic reticulum to Golgi vesicle-mediated transport (IMP)
- involved in protein geranylgeranylation (IDA)
- involved in protein targeting to membrane (IMP)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- part of Rab-protein geranylgeranyltransferase complex (IPI, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants are impaired in protein transport; reduced function mutants show decreased competitive fitness, are sensitive to DNA-damaging agent hydroxyurea (HU); heterozygous diploid nulls are haploinsufficient
Classical Genetics
- conditional
- null
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
444 total interactions for 357 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 4
- Biochemical Activity: 7
- Co-localization: 1
- Co-purification: 2
- FRET: 2
- Reconstituted Complex: 1
- Two-hybrid: 2
Genetic Interactions
- Negative Genetic: 377
- Positive Genetic: 37
- Synthetic Growth Defect: 1
- Synthetic Lethality: 6
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- BET2 encodes the beta subunit of the type II geranylgeranyltransferase (GGTase), a prenyltransferase that attaches geranylgeranyl groups to C-terminal cysteine residues of newly synthesized proteins. Bet2p forms a stable heterodimer with the alpha subunit Bet4p and together they function as the catalytic component of the enzyme. Another protein, Mrs6p, acts as an accessory subunit that delivers the substrates to the catalytic component. The primary purpose for this GGTase activity appears to be to add a membrane-attachment moiety to Ypt1p and Sec4p, two Rab-like, membrane-bound small GTPases involved in vesicular transport between the endoplasmic reticulum and the Golgi. All three subunits of the GGTase are therefore essential for the ER and Golgi functions, and for cell viability. Human homolog of BET2, RABGGTB, is associated with an inherited eye disease choroideremia, and is able to complement yeast bet2 mutants.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2010-05-18
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.