RAD51 / YER095W Overview
- Standard Name
- RAD51 1
- Systematic Name
- YER095W
- SGD ID
- SGD:S000000897
- Aliases
- MUT5 35
- Feature Type
- ORF , Verified
- Description
- Strand exchange protein; forms helical filament with DNA that searches for homology; involved in recombinational repair of DNA DSBs during vegetative growth and meiosis; phosphorylation by Cdc28p in G2/M phase promotes DNA binding, strand invasion, and primer extension; L1 and L2 motifs of DNA binding site I have critical roles in ensuring DNA fidelity during meiotic recombination; RAD51 and RAD4 pathways confer resistance to benzo[a]pyrene dihydrodiol; homolog of Dmc1p and bacterial RecA 2 3 4 5 6 7
- Name Description
- RADiation sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rad51p is 400 amino acids long, low in abundance, very short-lived; contains helix-hairpin-helix domain; sumoylated on K122, ubiquitinylated on K131, phosphorylated on 9 residues
- Length (a.a.)
- 400
- Mol. Weight (Da)
- 42943.9
- Isoelectric Point
- 4.54
- Median Abundance (molecules/cell)
- 6070 +/- 2766
- Half-life (hr)
- 3.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAD51 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Recombinase enzyme with DNA-dependent ATPase activity; involved in DNA recombination, telomere maintenance, mitochondrial double-strand break repair, and meiotic recombination; localizes to nuclear chromosomes and mitochondrial matrix
View computational annotations
Molecular Function
- Manually Curated
- enables ATP-dependent activity, acting on DNA (IDA)
- enables DNA binding (IDA)
- enables DNA strand exchange activity (IDA)
- enables double-stranded DNA binding (IDA, IMP)
- enables single-stranded DNA binding (IDA)
Biological Process
- Manually Curated
- involved in DNA recombination (IDA)
- involved in DNA strand invasion (IDA)
- involved in double-strand break repair (IMP)
- involved in G2/M transition of mitotic cell cycle (IDA)
- involved in heteroduplex formation (IDA)
- involved in meiotic joint molecule formation (IMP)
- involved in mitochondrial DNA repair (IMP)
- involved in nucleotide-excision repair (IMP)
- involved in reciprocal meiotic recombination (IMP)
- involved in telomere maintenance via recombination (IMP)
Cellular Component
- Manually Curated
- located in condensed nuclear chromosome (IDA)
- is active in mitochondrial chromosome (IDA)
- located in mitochondrial matrix (IDA)
- located in nuclear chromosome (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null displays increased chromosome instability, increased loss-of-heterozygosity in diploids, an increased mutation rate and elevated Ty element transposition; null mutant is temperature sensitive, and has both a decreased growth rate and competitive fitness on rich and synthetic media; null has decreased starvation resistance, increased thermotolerance, and decreased replicative lifespan that is increased relative to wt by caloric restriction; null contains large-budded cells with nuclear extensions at the nuclear envelope, adjacent to the nucleolus; homozygous null has a sporulation defect, and the heterozygous null is haploinsufficient
Classical Genetics
- null
- cell cycle progression: abnormal
- cellular morphology: abnormal
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- chronological lifespan: increased
- gamma ray resistance: decreased
- growth in exponential phase: decreased rate
- meiotic recombination: increased
- metal resistance: normal
- mitotic recombination: decreased
- mutation frequency: decreased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- radiation resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: decreased
- transposable element transposition: increased
- UV resistance: decreased
- reduction of function
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: increased
- innate thermotolerance: increased
- killer toxin resistance: decreased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- sporulation: decreased
- starvation resistance: decreased
- toxin resistance: decreased
- transposable element transposition: increased
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vegetative growth: decreased rate
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast RAD51 is homologous to human RAD51, and has been used to study primary ovarian insufficiency, colorectal and breast cancers
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rad51p interacts physically with proteins involved in DNA recombination and repair; RAD51 interacts genetically with genes involved in DNA repair
1380 total interactions for 549 unique genes
Physical Interactions
- Affinity Capture-MS: 49
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 33
- Biochemical Activity: 1
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 2
- Co-purification: 1
- PCA: 1
- Reconstituted Complex: 54
- Two-hybrid: 65
Genetic Interactions
- Dosage Growth Defect: 8
- Dosage Lethality: 3
- Dosage Rescue: 22
- Negative Genetic: 362
- Phenotypic Enhancement: 105
- Phenotypic Suppression: 115
- Positive Genetic: 92
- Synthetic Growth Defect: 230
- Synthetic Lethality: 135
- Synthetic Rescue: 91
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 13
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2001-01-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.