SDH1 / YKL148C Overview
- Standard Name
- SDH1 1 2
- Systematic Name
- YKL148C
- SGD ID
- SGD:S000001631
- Aliases
- SDHA 7
- Feature Type
- ORF , Verified
- Description
- Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; mutations in human ortholog SDHA are associated with Leigh syndrome and predisposition to several cancers 3 4 5 6
- Name Description
- Succinate DeHydrogenase 2
- Paralog
- SDH9
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SDH1/YKL148C is located on the left arm of chromosome XI between DBR1 RNA lariat debranching enzyme and AVT3 vacuolar transporter; coding sequence is 1923 nucleotides long with 7 SNPs, one of which causes a His/Tyr polymorphism at residue 202; SDH1 has a paralog, SDH9, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Sdh1p is 640 amino acids long, of average half-life, moderate in abundance; phosphorylated on S523, succinylated on 15 lysines
- Length (a.a.)
- 640
- Mol. Weight (Da)
- 70229.3
- Isoelectric Point
- 7.53
- Median Abundance (molecules/cell)
- 9672 +/- 3759
- Half-life (hr)
- 10.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SDH1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- FAD-binding succinate dehydrogenase subunit of the mitochondrial respiratory chain complex; involved in cellular respiration, the tricarboxylic acid cycle, and mitochondrial electron transport; localizes to the mitochondrial membrane
View computational annotations
Molecular Function
- Manually Curated
- enables flavin adenine dinucleotide binding (IMP, IDA)
- enables succinate dehydrogenase (quinone) activity (IMP, IDA, IGI)
Biological Process
- Manually Curated
- involved in cellular respiration (IMP, IGI)
- involved in mitochondrial electron transport, succinate to ubiquinone (IDA)
Cellular Component
- Manually Curated
- located in mitochondrial membrane (IDA)
- located in mitochondrion (HDA, IDA)
- part of respiratory chain complex II (succinate dehydrogenase) (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- The SDH1/YKL148C gene encodes a subunit of the succinate dehydrogenase complex, which plays a critical role in the tricarboxylic acid (TCA) cycle and electron transport chain in yeast. Mutants lacking the SDH1 function, specifically the null allele sdh1-Δ, exhibit a range of significant phenotypic abnormalities. These mutants are characterized by decreased anaerobic growth capability and abnormal cell cycle progression, indicating disruption in metabolic flexibility and growth regulation. Chemical compound accumulation is markedly altered in sdh1-Δ mutants, as they show increased levels of reactive oxygen species, alongside a decrease in the accumulation of certain compounds. The mutants exhibit increased excretion of various organic acids, including acetic, aconitic, succinic, and citric acids, suggesting metabolic dysregulation. Chronological lifespan is decreased in these mutants while replicative lifespan is increased. Null mutants also display reduced competitive fitness when exposed to ethanol, and decreased resistance to hyperosmotic and oxidative stress. SDH1 mutants cannot use alternative carbon sources like glycerol, lactate, or sodium acetate. Their ability of the null mutant to utilize nitrogen is also impaired, they grow slowly, and have abnormal vacuolar morphology.
Classical Genetics
- reduction of function
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- hyperosmotic stress resistance: decreased
- oxidative stress resistance: decreased
- petite
- protein/peptide accumulation: absent
- replicative lifespan: increased
- resistance to chemicals: decreased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory growth: decreased rate
- respiratory metabolism: decreased
- sporulation efficiency: decreased
- survival rate in stationary phase: decreased
- survival rate in stationary phase: increased
- toxin resistance: increased
- utilization of carbon source: absent
- overexpression
- null
- anaerobic growth: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: normal
- haploinsufficient
- protein/peptide accumulation: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- utilization of carbon source: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SDH1 is homologous to human SDHA, and has been used to study Leigh disease, mitochondrial complex II deficiency, late onset neurodegenerative disease, cardiomyopathy, leukodystrophy, paraganglioma, pheochromocytoma, pituitary adenoma, and gastrointestinal stromal tumor
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Sdh1p interacts physically with proteins involved in mitochondrion organization; SDH1 interacts genetically with genes involved in transcription; the sdh1 null mutant is viable, the null mutant of paralog sdh9 is viable, the sdh1 sdh9 double mutant displays a growth defect
199 total interactions for 145 unique genes
Physical Interactions
- Affinity Capture-MS: 35
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 9
- Co-localization: 1
- Cross-Linking-MS (XL-MS): 8
- Proximity Label-MS: 1
- Reconstituted Complex: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 2
- Negative Genetic: 100
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 5
- Positive Genetic: 22
- Synthetic Growth Defect: 5
- Synthetic Lethality: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-08
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.