SDH1 / YKL148C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

The SDH1/YKL148C gene encodes a subunit of the succinate dehydrogenase complex, which plays a critical role in the tricarboxylic acid (TCA) cycle and electron transport chain in yeast. Mutants lacking the SDH1 function, specifically the null allele sdh1-Δ, exhibit a range of significant phenotypic abnormalities. These mutants are characterized by decreased anaerobic growth capability and abnormal cell cycle progression, indicating disruption in metabolic flexibility and growth regulation. Chemical compound accumulation is markedly altered in sdh1-Δ mutants, as they show increased levels of reactive oxygen species, alongside a decrease in the accumulation of certain compounds. The mutants exhibit increased excretion of various organic acids, including acetic, aconitic, succinic, and citric acids, suggesting metabolic dysregulation. Chronological lifespan is decreased in these mutants while replicative lifespan is increased. Null mutants also display reduced competitive fitness when exposed to ethanol, and decreased resistance to hyperosmotic and oxidative stress. SDH1 mutants cannot use alternative carbon sources like glycerol, lactate, or sodium acetate. Their ability of the null mutant to utilize nitrogen is also impaired, they grow slowly, and have abnormal vacuolar morphology.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources