ARG82 / YDR173C Overview
- Standard Name
- ARG82 1
- Systematic Name
- YDR173C
- SGD ID
- SGD:S000002580
- Aliases
- ARGRIII 13 , ARGR3 21 , IPK2 5
- Feature Type
- ORF , Verified
- Description
- Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates genes responsive to arginine, phosphate, and nitrogen; needed by most [PSI+] variants for prion propagation 2 3 4 5 6 7 8
- Name Description
- ARGinine requiring
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 355
- Mol. Weight (Da)
- 40326.2
- Isoelectric Point
- 4.33
- Median Abundance (molecules/cell)
- 2720 +/- 1295
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ARG82 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phosphatidylinositol 3-kinase with broad substrate specificity; involved in inositol phosphate biosynthesis, regulation of arginine biosynthesis and transcription from RNA Pol II promoter; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables 1-phosphatidylinositol-3-kinase activity (IDA)
- enables inositol-1,3,4,5,6-pentakisphosphate kinase activity (IDA)
- enables inositol-1,3,4,5-tetrakisphosphate 6-kinase activity (IDA)
- enables inositol-1,4,5,6-tetrakisphosphate 3-kinase activity (IDA)
- enables inositol-1,4,5-trisphosphate 3-kinase activity (IMP)
- enables inositol-1,4,5-trisphosphate 6-kinase activity (IDA)
- enables protein-macromolecule adaptor activity (IPI, IMP)
Biological Process
- Manually Curated
- involved in inositol phosphate biosynthetic process (IDA)
- involved in macroautophagy (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in phosphatidylinositol phosphate biosynthetic process (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in protein stabilization (IMP)
- involved in regulation of arginine metabolic process (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sterile and auxotrophic for ornithine, arginine and myo-inositol; null mutant is heat sensitive, has a decreased growth rate on rich media, is unable to grow anaerobically and displays decreased competitive fitness on various media; null mutant is unable to utilize ornithine and has decreased ability to utilize ammonia as a nitrogen source; null mutant displays decreased autophagy, mitophagy, and has abnormal mitochondrial morphology; null mutant has decreased stress resistance; null mutant has altered inositol phosphate profiles; homozygous diploid null mutant has a G1 phase cell cycle delay, a strong sporulation defect, decreased glycogen accumulation and reduced desiccation resistance; heterozygous null mutant is haploproficient; overexpression results in a impaired mRNA export from the nucleus and enhanced invasive growth
Classical Genetics
- unspecified
- null
- autophagy: decreased
- auxotrophy
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- endocytosis: abnormal
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- invasive growth: decreased
- ionic stress resistance: decreased
- mitophagy: absent
- mitophagy: decreased
- nutrient uptake: increased
- pseudohyphal growth: decreased
- resistance to chemicals: decreased
- sporulation: absent
- sterile
- utilization of nitrogen source: absent
- utilization of nitrogen source: decreased
- overexpression
- reduction of function
- null
- auxotrophy
- biofilm formation: decreased
- cell cycle progression in G1 phase: delayed
- chemical compound accumulation: decreased
- cold sensitivity: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- haploproficient
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation: decreased
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased
- vacuolar morphology: abnormal
- vegetative growth: absent
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
59 total interactions for 46 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 3
- Reconstituted Complex: 4
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 3
- Negative Genetic: 20
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 2
- Positive Genetic: 4
- Synthetic Growth Defect: 4
- Synthetic Lethality: 2
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-01-22
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.